Intracellular Ca2+ homeostasis in rat round spermatids

JULIO ANDRES BERRIOS ARAYA, NELSON EDUARDO OSSES RIVERA, Carlos Opazo, Gloria Arenas, LUIS ALBERTO MERCADO VIANCO, Dale J. Benos, Juan G. Reyes

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Intracellular calcium, [Ca2+](i), can regulate meiotic progression of mammalian oocytes. However, the role of [Ca2+](i) in the regulation of the spermatogenic process and its cellular homeostatic mechanisms in spermatogenic cells has not been elucidated. Using intracellular fluorescent probes for Ca2+ and immunodetection of plasma membrane (PM) Ca2+-ATPases, we report that: a) rat round spermatids maintain [Ca2+](i) levels of 60 ± 5 nM (SEM), as estimated with fluo-3 in single cells or fura-2 in cells in suspension; b) these cells regulate [Ca2+](i), by actively extruding it using a PM Ca2+-ATPase; c) rat spermatids also actively transport Ca2+ by sarco-endoplasmic reticulum type ATPases (SERCA); d) rat spermatids possess non-mitochondrial intracellular Ca2+(i) stores insensitive to thapsigargin but releasable by ionomycin; and e) rat spermatids do not activate Ca2+ entry mechanisms by the release of Ca2+ from SERCA-regulated stores. These results demonstrate that rat round spermatids can generate modulated intracellular Ca2+ signals upon activation of Ca2+ channels or Ca2+ release from intracellular stores.

Original languageEnglish
Pages (from-to)391-398
Number of pages8
JournalBiology of the Cell
Volume90
Issue number5
DOIs
StatePublished - 1 Jan 1998

Keywords

  • Cell differentiation
  • Seminiferous tubules
  • Spermatogenesis
  • Testis

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