TY - JOUR
T1 - Isolation and characterization of an Antarctic Flavobacterium strain with agarase and alginate lyase activities
AU - Lavín, Paris
AU - Atala, Cristian
AU - Gallardo-Cerda, Jorge
AU - Gonzalez-Aravena, Marcelo
AU - De La Iglesia, Rodrigo
AU - Oses, Rómulo
AU - Torres-Díaz, Cristian
AU - Trefault, Nicole
AU - Molina-Montenegro, Marco A.
AU - Laughinghouse, H. Dail
N1 - Publisher Copyright:
© Polish Academy of Sciences 2016.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17°C) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures.
AB - Several bacteria that are associated with macroalgae can use phycocolloids as a carbon source. Strain INACH002, isolated from decomposing Porphyra (Rhodophyta), in King George Island, Antarctica, was screened and characterized for the ability to produce agarase and alginate-lyase enzymatic activities. Our strain INACH002 was identified as a member of the genus Flavobacterium, closely related to Flavobacterium faecale, using 16S rRNA gene analysis. The INACH002 strain was characterized as psychrotrophic due to its optimal temperature (17°C) and maximum temperature (20°C) of growth. Agarase and alginate-lyase displayed enzymatic activities within a range of 10°C to 50°C, with differences in the optimal temperature to hydrolyze agar (50°C), agarose (50°C) and alginate (30°C) during the first 30 min of activity. Strain Flavobacterium INACH002 is a promising Antarctic biotechnological resource; however, further research is required to illustrate the structural and functional bases of the enzymatic performance observed during the degradation of different substrates at different temperatures.
KW - Antarctic
KW - Flavobacterium
KW - King George Island
KW - agarase
KW - alginate-lyase
UR - http://www.scopus.com/inward/record.url?scp=84992093453&partnerID=8YFLogxK
U2 - 10.1515/popore-2016-0021
DO - 10.1515/popore-2016-0021
M3 - Article
AN - SCOPUS:84992093453
SN - 0138-0338
VL - 37
SP - 403
EP - 419
JO - Polish Polar Research
JF - Polish Polar Research
IS - 3
ER -