TY - JOUR
T1 - Key microbial populations involved in anaerobic degradation of phenol and p-cresol using different inocula
AU - Franchi, Oscar
AU - Rosenkranz, Francisca
AU - Chamy, Rolando
N1 - Funding Information:
This study was supported by CONICYT Project 781302010 and FONDECYT Project 1151161 from CONICYT-CHILE.
Funding Information:
This study was supported by CONICYT Project 781302010 and FONDECYT Project 1151161 from CONICYT-CHILE.
Publisher Copyright:
© 2018
PY - 2018/9
Y1 - 2018/9
N2 - Background: Anaerobic digestion is an alternative bioprocess used to treat effluents containing toxic compounds such as phenol and p-cresol. Selection of an adequate sludge as inoculum containing an adapted microbial consortium is a relevant factor to improve the removal of these pollutants. The objective of this study is to identify the key microorganisms involved in the anaerobic digestion of phenol and p-cresol and elucidate the relevance of the bamA gene abundance (a marker gene for aromatic degraders) in the process, in order to establish new strategies for inocula selection and improve the system's performance. Results: Successive batch anaerobic digestion of phenol and p-cresol was performed using granular or suspended sludge. Granular sludge in comparison to suspended sludge showed higher degradation rates both for phenol (11.3 ± 0.7 vs 8.1 ± 1.1 mg l-1 d-1) and p-cresol (7.8 ± 0.4 vs 3.7 ± 1.0 mg l-1 d-1). After three and four re-feedings of phenol and p-cresol, respectively, the microbial structure from both sludges was clearly different from the original sludges. Anaerobic digestion of phenol and p-cresol generated an abundance increase in Syntrophorhabdus genus and bamA gene, together with hydrogenotrophic and aceticlastic archaea. Analysis of results indicates that differences in methanogenic pathways and levels of Syntrophorhabdus and bamA gene in the inocula, could be the causes of dissimilar degradation rates between each sludge. Conclusions: Syntrophorhabdus and bamA gene play relevant roles in anaerobic degradation of phenolics. Estimation of these components could serve as a fast screening tool to find the most acclimatized sludge to efficiently degrade mono-aromatic compounds. How to cite: Franchi O, Rosenkranz F, Chamy R. Key microbial populations involved in anaerobic degradation of phenol and p-cresol using different inocula. Electron J Biotechnol 2018;35. https://doi.org/10.1016/j.ejbt.2018.08.002.
AB - Background: Anaerobic digestion is an alternative bioprocess used to treat effluents containing toxic compounds such as phenol and p-cresol. Selection of an adequate sludge as inoculum containing an adapted microbial consortium is a relevant factor to improve the removal of these pollutants. The objective of this study is to identify the key microorganisms involved in the anaerobic digestion of phenol and p-cresol and elucidate the relevance of the bamA gene abundance (a marker gene for aromatic degraders) in the process, in order to establish new strategies for inocula selection and improve the system's performance. Results: Successive batch anaerobic digestion of phenol and p-cresol was performed using granular or suspended sludge. Granular sludge in comparison to suspended sludge showed higher degradation rates both for phenol (11.3 ± 0.7 vs 8.1 ± 1.1 mg l-1 d-1) and p-cresol (7.8 ± 0.4 vs 3.7 ± 1.0 mg l-1 d-1). After three and four re-feedings of phenol and p-cresol, respectively, the microbial structure from both sludges was clearly different from the original sludges. Anaerobic digestion of phenol and p-cresol generated an abundance increase in Syntrophorhabdus genus and bamA gene, together with hydrogenotrophic and aceticlastic archaea. Analysis of results indicates that differences in methanogenic pathways and levels of Syntrophorhabdus and bamA gene in the inocula, could be the causes of dissimilar degradation rates between each sludge. Conclusions: Syntrophorhabdus and bamA gene play relevant roles in anaerobic degradation of phenolics. Estimation of these components could serve as a fast screening tool to find the most acclimatized sludge to efficiently degrade mono-aromatic compounds. How to cite: Franchi O, Rosenkranz F, Chamy R. Key microbial populations involved in anaerobic degradation of phenol and p-cresol using different inocula. Electron J Biotechnol 2018;35. https://doi.org/10.1016/j.ejbt.2018.08.002.
KW - Anaerobic digestion
KW - Aromatic degraders
KW - Degradation of p-cresol
KW - Degradation of phenol
KW - Illumina
KW - Microbial consortium
KW - Phenolics
KW - Sludge
KW - Syntrophorhabdus
KW - bamA gene
KW - qPCR
UR - http://www.scopus.com/inward/record.url?scp=85053003843&partnerID=8YFLogxK
U2 - 10.1016/j.ejbt.2018.08.002
DO - 10.1016/j.ejbt.2018.08.002
M3 - Article
AN - SCOPUS:85053003843
VL - 35
SP - 33
EP - 38
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
SN - 0717-3458
ER -