Kinetics, molecular basis, and differentiation of L-lactate transport in spermatogenic cells

Sebastian Brauchi, Maria C. Rauch, Ivan E. Alfaro, Christian Cea, Ilona I. Concha, Dale J. Benos, JUAN GUILLERMO REYES MARTINEZ

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Round spermatid energy metabolism is closely dependent on the presence of L-lactate in the external medium. This L-lactate has been proposed to be supplied by Sertoli cells in the seminiferous tubules. L-Lactate, in conjunction with glucose, modulates intracellular Ca2+ concentration in round spermatids and pachytene spermatocytes. In spite of this central role of L-lactate in spermatogenic cell physiology, the mechanism of L-lactate transport, as well as possible differentiation during spermatogenesis, has not been studied in these cells. By measuring radioactive L-lactate transport and intracellular pH (pHi) changes with pHi fluorescent probes, we show that these cells transport L-lactate using monocarboxylate- H+ transport (MCT) systems. RT-PCR, in situ mRNA hybridization, and immunocyto- and immunohistochemistry data show that pachytene spermatocytes express mainly the MCT1 and MCT4 isoforms of the transporter (intermediate- and low-affinity transporters, respectively), while round spermatids, besides MCT1 and MCT4, also show expression of the MCT2 isoform (high-affinity transporter). These molecular data are consistent with the kinetic data of L-lactate transport in these cells demonstrating at least two transport components for L-lactate. These separate transport components reflect the ability of these cells to switch between the generation of glycolytic L-lactate in the presence of external glucose and the use of L-lactate when this substrate is available in the external environment. The supply of these substrates is regulated by the hormonal control of Sertoli cell glycolytic activity.

Original languageEnglish
Pages (from-to)C523-C534
JournalAmerican Journal of Physiology - Cell Physiology
Volume288
Issue number3 57-3
DOIs
StatePublished - Mar 2005
Externally publishedYes

Keywords

  • Cell differentiation
  • Meiosis
  • Seminiferous tubules
  • Spermatogenesis
  • Testicle

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