Kinetics of ABE fermentation considering the different phenotypes present in a batch culture of Clostridium beijerinckii NCIMB-8052

Iván Paredes, Julián Quintero, Karlo Guerrero, Roberto Gallardo, Silvia Mau, Raúl Conejeros, Juan Carlos Gentina, German Aroca

Research output: Contribution to journalArticlepeer-review

Abstract

Background: In solvent-producing Clostridium species, several physiological conditions and morphological forms can be observed during batch ABE fermentation. Currently, the contribution made by each condition or form to the production of acids and solvents is not clear. Flow cytometry and fluorescence microscopy have been used to analyze the presence of different morphological and physiological conditions for Clostridium species; however, there are few studies to quantify their contribution to fermentation kinetics. Results: Morphological changes of the batch cultures of Clostridium beijerinckii NCIMB-8052 were followed by flow cytometry and epifluorescence microscopy. Three cell types were identified, and the percentage of each one was estimated along the culture, showing that they are present throughout the culture and its population distribution was in agreement with the kinetics of growth and acids and solvents production. Yields for the global population and for each subpopulation were estimated. The values of the yield for butanol and acetone based on the fraction of solventogenic cells were 2.5 times the values of the parameters obtained for the whole biomass, while those for acetic and butyric acids based on the acidogenic cell type was 1.5 times. Conclusion: Quantitative analysis of the subpopulations by flow cytometry allows the calculation of the yields related to specific cell types, isolating the contribution of each subpopulation to the production of acids and solvents. How to cite: Paredes I, Quintero J, Guerrero K, et al. Kinetics of ABE fermentation considering the different phenotypes present in a batch culture of Clostridium beijerinckii NCIMB-8052. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2021.12.002

Original languageEnglish
Pages (from-to)12-21
Number of pages10
JournalElectronic Journal of Biotechnology
Volume56
DOIs
StatePublished - Mar 2022
Externally publishedYes

Keywords

  • ABE fermentation
  • Acidogenic
  • Batch cultures
  • Butanol production
  • Clostridium
  • Clostridium beijerinckii
  • Fermentation kinetics
  • Flow cytometry
  • Fluorescence microscopy
  • Solventogenic
  • Solvents production

Fingerprint

Dive into the research topics of 'Kinetics of ABE fermentation considering the different phenotypes present in a batch culture of Clostridium beijerinckii NCIMB-8052'. Together they form a unique fingerprint.

Cite this