TY - JOUR
T1 - Kinetics of ABE fermentation considering the different phenotypes present in a batch culture of Clostridium beijerinckii NCIMB-8052
AU - Paredes, Iván
AU - Quintero, Julián
AU - Guerrero, Karlo
AU - Gallardo, Roberto
AU - Mau, Silvia
AU - Conejeros, Raúl
AU - Gentina, Juan Carlos
AU - Aroca, German
N1 - Funding Information:
This work was financed by the National Research and Development Agency (ANID, former CONICYT) through Projects FONDECYT 21151422 , and FONDECYT 3140232 , Project SMIBIO ERANET-LAC ELAC2014/BEE0249, and the Pontificia Universidad Católica de Valparaíso.
Funding Information:
This work was financed by the National Research and Development Agency (ANID, former CONICYT) through Projects FONDECYT 21151422, and FONDECYT 3140232, Project SMIBIO ERANET-LAC ELAC2014/BEE0249, and the Pontificia Universidad Cat?lica de Valpara?so.
Publisher Copyright:
© 2022
PY - 2022/3
Y1 - 2022/3
N2 - Background: In solvent-producing Clostridium species, several physiological conditions and morphological forms can be observed during batch ABE fermentation. Currently, the contribution made by each condition or form to the production of acids and solvents is not clear. Flow cytometry and fluorescence microscopy have been used to analyze the presence of different morphological and physiological conditions for Clostridium species; however, there are few studies to quantify their contribution to fermentation kinetics. Results: Morphological changes of the batch cultures of Clostridium beijerinckii NCIMB-8052 were followed by flow cytometry and epifluorescence microscopy. Three cell types were identified, and the percentage of each one was estimated along the culture, showing that they are present throughout the culture and its population distribution was in agreement with the kinetics of growth and acids and solvents production. Yields for the global population and for each subpopulation were estimated. The values of the yield for butanol and acetone based on the fraction of solventogenic cells were 2.5 times the values of the parameters obtained for the whole biomass, while those for acetic and butyric acids based on the acidogenic cell type was 1.5 times. Conclusion: Quantitative analysis of the subpopulations by flow cytometry allows the calculation of the yields related to specific cell types, isolating the contribution of each subpopulation to the production of acids and solvents. How to cite: Paredes I, Quintero J, Guerrero K, et al. Kinetics of ABE fermentation considering the different phenotypes present in a batch culture of Clostridium beijerinckii NCIMB-8052. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2021.12.002
AB - Background: In solvent-producing Clostridium species, several physiological conditions and morphological forms can be observed during batch ABE fermentation. Currently, the contribution made by each condition or form to the production of acids and solvents is not clear. Flow cytometry and fluorescence microscopy have been used to analyze the presence of different morphological and physiological conditions for Clostridium species; however, there are few studies to quantify their contribution to fermentation kinetics. Results: Morphological changes of the batch cultures of Clostridium beijerinckii NCIMB-8052 were followed by flow cytometry and epifluorescence microscopy. Three cell types were identified, and the percentage of each one was estimated along the culture, showing that they are present throughout the culture and its population distribution was in agreement with the kinetics of growth and acids and solvents production. Yields for the global population and for each subpopulation were estimated. The values of the yield for butanol and acetone based on the fraction of solventogenic cells were 2.5 times the values of the parameters obtained for the whole biomass, while those for acetic and butyric acids based on the acidogenic cell type was 1.5 times. Conclusion: Quantitative analysis of the subpopulations by flow cytometry allows the calculation of the yields related to specific cell types, isolating the contribution of each subpopulation to the production of acids and solvents. How to cite: Paredes I, Quintero J, Guerrero K, et al. Kinetics of ABE fermentation considering the different phenotypes present in a batch culture of Clostridium beijerinckii NCIMB-8052. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2021.12.002
KW - ABE fermentation
KW - Acidogenic
KW - Batch cultures
KW - Butanol production
KW - Clostridium
KW - Clostridium beijerinckii
KW - Fermentation kinetics
KW - Flow cytometry
KW - Fluorescence microscopy
KW - Solventogenic
KW - Solvents production
UR - http://www.scopus.com/inward/record.url?scp=85123849055&partnerID=8YFLogxK
U2 - 10.1016/j.ejbt.2021.12.002
DO - 10.1016/j.ejbt.2021.12.002
M3 - Article
AN - SCOPUS:85123849055
VL - 56
SP - 12
EP - 21
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
SN - 0717-3458
ER -