TY - JOUR
T1 - Lipase-Catalyzed Solvent-Free Transesterification of Wood Sterols
AU - Martínez, Irene
AU - Markovits, Alejandro
AU - Chamy, Rolando
AU - Markovits, Andrés
N1 - Funding Information:
This work was financed by FONTEC, Project no. 199-1934, Chile.
PY - 2004/1
Y1 - 2004/1
N2 - Eighteen commercial lipase preparations, either immobilized or crude enzyme powders, were screened for the transesterification of wood sterols. The reactions were carried out in a solvent-free system, at the optimum temperature of the enzyme preparations as reported by the manufacturer and at the pressure of 2 mbar, with 5 or 10% in weight of the enzyme relative to the wood sterol content of the reacting mixture. Methyl esters of sunflower fatty acids were used as transesterifying agent. Of all the enzymes assayed, only Lipase TL from Pseudomonas stutzeri PL-836 (Meito Sangyo) exhibited any significant transesterifying capacity, 85 and 95% of conversion after 2 and 8 h of reaction, respectively, when 10% in weight of enzyme was used.
AB - Eighteen commercial lipase preparations, either immobilized or crude enzyme powders, were screened for the transesterification of wood sterols. The reactions were carried out in a solvent-free system, at the optimum temperature of the enzyme preparations as reported by the manufacturer and at the pressure of 2 mbar, with 5 or 10% in weight of the enzyme relative to the wood sterol content of the reacting mixture. Methyl esters of sunflower fatty acids were used as transesterifying agent. Of all the enzymes assayed, only Lipase TL from Pseudomonas stutzeri PL-836 (Meito Sangyo) exhibited any significant transesterifying capacity, 85 and 95% of conversion after 2 and 8 h of reaction, respectively, when 10% in weight of enzyme was used.
KW - Enzymatic transesterification
KW - Lipase
KW - Solvent-free system
KW - Sterols
KW - Steryl esters
UR - http://www.scopus.com/inward/record.url?scp=0842346885&partnerID=8YFLogxK
U2 - 10.1385/ABAB:112:1:55
DO - 10.1385/ABAB:112:1:55
M3 - Article
C2 - 14734838
AN - SCOPUS:0842346885
SN - 0273-2289
VL - 112
SP - 55
EP - 62
JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
IS - 1
ER -