Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infections

Natanael Dantas Farias, Marcelo Falchetti, Gabriel Machado Matos, PAULINA SCHMITT RIVERA, Cairé Barreto, Nicolas Argenta, Jean Luc Rolland, Evelyne Bachère, Luciane Maria Perazzolo, Rafael Diego Rosa

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. vannamei stylicin family is composed of two members (Lvan-Stylicin1 and Lvan-Stylicin2) encoded by different loci which vary in gene copy number. Unlike the other three gene-encoded antimicrobial peptide families from penaeid shrimp, the expression of Lvan-Stylicins is not restricted to hemocytes. Indeed, they are also produced by the columnar epithelial cells lining the midgut and its anterior caecum. Interestingly, Lvan-Stylicins are simultaneously transcribed at different transcriptional levels in a single shrimp and are differentially modulated in hemocytes after infections. While the expression of both genes showed to be responsive to damage-associated molecular patterns, only Lvan-Stylicin2 was induced after a Vibrio infection. Besides, Lvan-Stylicins also showed a distinct pattern of gene expression in the three portions of the midgut (anterior, middle and posterior) and during shrimp development. We provide here the first evidence of the diversity of the stylicin antimicrobial peptide family in terms of sequence and gene expression distribution and regulation.

Original languageEnglish
Pages (from-to)82-92
Number of pages11
JournalFish and Shellfish Immunology
Volume86
DOIs
StatePublished - 1 Mar 2019

Keywords

  • Antimicrobial peptide
  • Crustacean
  • Host defense peptide
  • Invertebrate immunity
  • Molecular diversity
  • Penaeid shrimp

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