Low-Cost Fourier Ptychography Microscope Kit

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present an advanced low-cost wide-field, high-resolution computational microscope kit based on Fourier ptychography. The microscope is integrated by using a collection of easy to find off-the-shelf components. By implementing the Fourier Ptychographic Microscopy (FPM) technique, we are able to acquire and synthetically produce wide field-of-view images at high resolutions, exceeding the original limitations of the optical system. The system works by iteratively updating a mosaiced high resolution Fourier spectrum from a collection of images of a sample captured at different illumination angles. We provide with the design of the low-cost kit and also with the results obtained by several tests performed with a calibration target and blood samples. From the results, the increase in resolution of the system is quantitatively and qualitatively demonstrated, while preserving the original wide field-of-view of the objective lens used.

Original languageEnglish
Title of host publication2019 22nd Symposium on Image, Signal Processing and Artificial Vision, STSIVA 2019 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728114910
DOIs
StatePublished - 1 Apr 2019
Event22nd Symposium on Image, Signal Processing and Artificial Vision, STSIVA 2019 - Bucaramanga, Colombia
Duration: 24 Apr 201926 Apr 2019

Publication series

Name2019 22nd Symposium on Image, Signal Processing and Artificial Vision, STSIVA 2019 - Conference Proceedings

Conference

Conference22nd Symposium on Image, Signal Processing and Artificial Vision, STSIVA 2019
CountryColombia
CityBucaramanga
Period24/04/1926/04/19

Keywords

  • computational imaging
  • Fourier ptychography
  • microscopy
  • phase retrieval

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