TY - GEN
T1 - Low-Cost Fourier Ptychography Microscope Kit
AU - Guzmán, Felipe
AU - VERA ROJAS, ESTEBAN MAURICIO
PY - 2019/4/1
Y1 - 2019/4/1
N2 - We present an advanced low-cost wide-field, high-resolution computational microscope kit based on Fourier ptychography. The microscope is integrated by using a collection of easy to find off-the-shelf components. By implementing the Fourier Ptychographic Microscopy (FPM) technique, we are able to acquire and synthetically produce wide field-of-view images at high resolutions, exceeding the original limitations of the optical system. The system works by iteratively updating a mosaiced high resolution Fourier spectrum from a collection of images of a sample captured at different illumination angles. We provide with the design of the low-cost kit and also with the results obtained by several tests performed with a calibration target and blood samples. From the results, the increase in resolution of the system is quantitatively and qualitatively demonstrated, while preserving the original wide field-of-view of the objective lens used.
AB - We present an advanced low-cost wide-field, high-resolution computational microscope kit based on Fourier ptychography. The microscope is integrated by using a collection of easy to find off-the-shelf components. By implementing the Fourier Ptychographic Microscopy (FPM) technique, we are able to acquire and synthetically produce wide field-of-view images at high resolutions, exceeding the original limitations of the optical system. The system works by iteratively updating a mosaiced high resolution Fourier spectrum from a collection of images of a sample captured at different illumination angles. We provide with the design of the low-cost kit and also with the results obtained by several tests performed with a calibration target and blood samples. From the results, the increase in resolution of the system is quantitatively and qualitatively demonstrated, while preserving the original wide field-of-view of the objective lens used.
KW - computational imaging
KW - Fourier ptychography
KW - microscopy
KW - phase retrieval
UR - http://www.scopus.com/inward/record.url?scp=85068025508&partnerID=8YFLogxK
U2 - 10.1109/STSIVA.2019.8730237
DO - 10.1109/STSIVA.2019.8730237
M3 - Conference contribution
T3 - 2019 22nd Symposium on Image, Signal Processing and Artificial Vision, STSIVA 2019 - Conference Proceedings
BT - 2019 22nd Symposium on Image, Signal Processing and Artificial Vision, STSIVA 2019 - Conference Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 22nd Symposium on Image, Signal Processing and Artificial Vision, STSIVA 2019
Y2 - 24 April 2019 through 26 April 2019
ER -