Massive gene expansion and sequence diversification is associated with diverse tissue distribution, regulation and antimicrobial properties of anti-lipopolysaccharide factors in shrimp

Gabriel Machado Matos, PAULINA SCHMITT RIVERA, Cairé Barreto, Natanael Dantas Farias, Guilherme Toledo-Silva, Fanny Guzmán, Delphine Destoumieux-Garzón, Luciane Maria Perazzolo, Rafael Diego Rosa

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides with a central β-hairpin structure able to bind to microbial components. Mining sequence databases for ALFs allowed us to show the remarkable diversity of ALF sequences in shrimp. We found at least seven members of the ALF family (Groups A to G), including two novel Groups (F and G), all of which are encoded by different loci with conserved gene organization. Phylogenetic analyses revealed that gene expansion and subsequent diversification of the ALF family occurred in crustaceans before shrimp speciation occurred. The transcriptional profile of ALFs was compared in terms of tissue distribution, response to two pathogens and during shrimp development in Litopenaeus vannamei, the most cultivated species. ALFs were found to be constitutively expressed in hemocytes and to respond differently to tissue damage. While synthetic β-hairpins of Groups E and G displayed both antibacterial and antifungal activities, no activity was recorded for Group F β-hairpins. Altogether, our results showed that ALFs form a family of shrimp AMPs that has been the subject of intense diversification. The different genes differ in terms of tissue expression, regulation and function. These data strongly suggest that multiple selection pressures have led to functional diversification of ALFs in shrimp.

Original languageEnglish
Article number381
JournalMarine Drugs
Volume16
Issue number10
DOIs
StatePublished - 11 Oct 2018

Keywords

  • Anti-LPS factor
  • Antimicrobial activity
  • Antimicrobial peptide
  • Crustacean
  • Functional diversity
  • Host defense peptide
  • Host-microbe relationship
  • Invertebrate immunity

Fingerprint Dive into the research topics of 'Massive gene expansion and sequence diversification is associated with diverse tissue distribution, regulation and antimicrobial properties of anti-lipopolysaccharide factors in shrimp'. Together they form a unique fingerprint.

Cite this