Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Cristian Acevedo; Matthias H. Stach; Anette Amtmann; Manuel E. Young; Juan G. Reyes; Holger Huebner; Rainer Buchholz

doi:10.2225/vol12-issue2-fulltext-10

Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Cristian Acevedo, Matthias H. Stach, Anette Amtmann, Manuel E. Young, Juan G. Reyes, Holger Huebner, Rainer Buchholz

INSTITUTE OF CHEMISTRY

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3 Scopus citations

Abstract

The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

Original language	English
Journal	Electronic Journal of Biotechnology
Volume	12
Issue number	2
DOIs	https://doi.org/10.2225/vol12-issue2-fulltext-10
State	Published - 15 Apr 2009

Keywords

Delta milli pH
Differential pH
β-Galactosidase

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abstract = "The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.",

keywords = "Delta milli pH, Differential pH, β-Galactosidase",

author = "Cristian Acevedo and Stach, {Matthias H.} and Anette Amtmann and Young, {Manuel E.} and Reyes, {Juan G.} and Holger Huebner and Rainer Buchholz",

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AU - Acevedo, Cristian

AU - Stach, Matthias H.

AU - Amtmann, Anette

AU - Young, Manuel E.

AU - Reyes, Juan G.

AU - Huebner, Holger

AU - Buchholz, Rainer

PY - 2009/4/15

Y1 - 2009/4/15

N2 - The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

AB - The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

KW - Delta milli pH

KW - Differential pH

KW - β-Galactosidase

UR - http://www.scopus.com/inward/record.url?scp=66849142198&partnerID=8YFLogxK

U2 - 10.2225/vol12-issue2-fulltext-10

DO - 10.2225/vol12-issue2-fulltext-10

M3 - Article

AN - SCOPUS:66849142198

SN - 0717-3458

VL - 12

JO - Electronic Journal of Biotechnology

JF - Electronic Journal of Biotechnology

IS - 2

ER -

Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Abstract

Keywords

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