Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Cristian Acevedo, Matthias H. Stach, Anette Amtmann, Manuel E. Young, JUAN GUILLERMO REYES MARTINEZ, Holger Huebner, Rainer Buchholz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

Original languageEnglish
JournalElectronic Journal of Biotechnology
Volume12
Issue number2
DOIs
StatePublished - 15 Apr 2009
Externally publishedYes

Keywords

  • β-Galactosidase
  • Delta milli pH
  • Differential pH

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