Mechanisms underlying the toxic effects of antimony species in human embryonic kidney cells (HEK-293) and their comparison with arsenic species

Marcelo Verdugo, Yasumitsu Ogra, WALDO EMERSON QUIROZ VENEGAS

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Antimony cytotoxicity was assessed in human embryonic kidney cells (HEK-293). Uptake, mitochondrial respiratory activity, ROS generation and diffusional kinetics were measured using fluorescence recovery after photobleaching (FRAP). Furthermore, the toxic effect induced by Sb was compared with As toxicity in regard to ROS generation and diffusional kinetics, which provides information on the protein aggregation process. Our results show a favored uptake of Sb(III) and a more severe effect, decreasing the mitochondrial activity more than in the presence of Sb(V). In comparison with As, the Sb species did not generate a significant increase in ROS generation, which was observed with As(III) and As(V). FRAP analysis yielded important information on the diffusion and binding dynamics of live cells in presence of these metalloids. The mobile fraction showed a strong decrease with the As species and Sb(III). The diffusion rate and the koff-rate were significantly decreased for the As and Sb species but were more strong in the presence of As(III).

Original languageEnglish
Pages (from-to)783-792
Number of pages10
JournalJournal of Toxicological Sciences
Volume41
Issue number6
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • Antimony toxicity
  • Arsenic toxicity
  • FRAP
  • Protein aggregation
  • Sb uptake

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