Mineralocorticoids modulate the expression of the β-3 subunit of the Na+, K+-ATPase in the renal collecting duct

Macarena Rojas; Pablo Díaz; Pablo León; Alexis A. Gonzalez; Magdalena González; Víctor Barrientos; Nikolay B. Pestov; Rodrigo Alzamora; Luis Michea

doi:10.1080/19336950.2017.1344800

Mineralocorticoids modulate the expression of the β-3 subunit of the Na⁺, K⁺-ATPase in the renal collecting duct

Macarena Rojas, Pablo Díaz, Pablo León, Alexis A. Gonzalez, Magdalena González, Víctor Barrientos, Nikolay B. Pestov, Rodrigo Alzamora, Luis Michea

INSTITUTE OF CHEMISTRY

Research output: Contribution to journal › Article › peer-review

Abstract

Renal sodium reabsorption depends on the activity of the Na⁺,K⁺-ATPase α/β heterodimer. Four α (α_1–4) and 3 β (β_1–3) subunit isoforms have been described. It is accepted that renal tubule cells express α₁/β₁ dimers. Aldosterone stimulates Na⁺,K⁺-ATPase activity and may modulate α₁/β₁ expression. However, some studies suggest the presence of β₃ in the kidney. We hypothesized that the β₃ isoform of the Na⁺,K⁺-ATPase is expressed in tubular cells of the distal nephron, and modulated by mineralocorticoids. We found that β₃ is highly expressed in collecting duct of rodents, and that mineralocorticoids decreased the expression of β₃. Thus, we describe a novel molecular mechanism of sodium pump modulation that may contribute to the effects of mineralocorticoids on sodium reabsorption.

Original language	English
Pages (from-to)	388-398
Number of pages	11
Journal	Channels
Volume	11
Issue number	5
DOIs	https://doi.org/10.1080/19336950.2017.1344800
State	Published - 3 Sep 2017

Keywords

aldosterone
epithelial sodium channel
hypertension
intercalated cells
pendrin
principal cells
sodium pump

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10.1080/19336950.2017.1344800

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title = "Mineralocorticoids modulate the expression of the β-3 subunit of the Na+, K+-ATPase in the renal collecting duct",

abstract = "Renal sodium reabsorption depends on the activity of the Na+,K+-ATPase α/β heterodimer. Four α (α1–4) and 3 β (β1–3) subunit isoforms have been described. It is accepted that renal tubule cells express α1/β1 dimers. Aldosterone stimulates Na+,K+-ATPase activity and may modulate α1/β1 expression. However, some studies suggest the presence of β3 in the kidney. We hypothesized that the β3 isoform of the Na+,K+-ATPase is expressed in tubular cells of the distal nephron, and modulated by mineralocorticoids. We found that β3 is highly expressed in collecting duct of rodents, and that mineralocorticoids decreased the expression of β3. Thus, we describe a novel molecular mechanism of sodium pump modulation that may contribute to the effects of mineralocorticoids on sodium reabsorption.",

keywords = "aldosterone, epithelial sodium channel, hypertension, intercalated cells, pendrin, principal cells, sodium pump",

author = "Macarena Rojas and Pablo D{\'i}az and Pablo Le{\'o}n and Gonzalez, {Alexis A.} and Magdalena Gonz{\'a}lez and V{\'i}ctor Barrientos and Pestov, {Nikolay B.} and Rodrigo Alzamora and Luis Michea",

note = "Publisher Copyright: {\textcopyright} 2017 Taylor & Francis.",

year = "2017",

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doi = "10.1080/19336950.2017.1344800",

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T1 - Mineralocorticoids modulate the expression of the β-3 subunit of the Na+, K+-ATPase in the renal collecting duct

AU - Rojas, Macarena

AU - Díaz, Pablo

AU - León, Pablo

AU - Gonzalez, Alexis A.

AU - González, Magdalena

AU - Barrientos, Víctor

AU - Pestov, Nikolay B.

AU - Alzamora, Rodrigo

AU - Michea, Luis

PY - 2017/9/3

Y1 - 2017/9/3

N2 - Renal sodium reabsorption depends on the activity of the Na+,K+-ATPase α/β heterodimer. Four α (α1–4) and 3 β (β1–3) subunit isoforms have been described. It is accepted that renal tubule cells express α1/β1 dimers. Aldosterone stimulates Na+,K+-ATPase activity and may modulate α1/β1 expression. However, some studies suggest the presence of β3 in the kidney. We hypothesized that the β3 isoform of the Na+,K+-ATPase is expressed in tubular cells of the distal nephron, and modulated by mineralocorticoids. We found that β3 is highly expressed in collecting duct of rodents, and that mineralocorticoids decreased the expression of β3. Thus, we describe a novel molecular mechanism of sodium pump modulation that may contribute to the effects of mineralocorticoids on sodium reabsorption.

AB - Renal sodium reabsorption depends on the activity of the Na+,K+-ATPase α/β heterodimer. Four α (α1–4) and 3 β (β1–3) subunit isoforms have been described. It is accepted that renal tubule cells express α1/β1 dimers. Aldosterone stimulates Na+,K+-ATPase activity and may modulate α1/β1 expression. However, some studies suggest the presence of β3 in the kidney. We hypothesized that the β3 isoform of the Na+,K+-ATPase is expressed in tubular cells of the distal nephron, and modulated by mineralocorticoids. We found that β3 is highly expressed in collecting duct of rodents, and that mineralocorticoids decreased the expression of β3. Thus, we describe a novel molecular mechanism of sodium pump modulation that may contribute to the effects of mineralocorticoids on sodium reabsorption.

KW - aldosterone

KW - epithelial sodium channel

KW - hypertension

KW - intercalated cells

KW - pendrin

KW - principal cells

KW - sodium pump

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DO - 10.1080/19336950.2017.1344800

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AN - SCOPUS:85025121386

SN - 1933-6950

VL - 11

SP - 388

EP - 398

JO - Channels

JF - Channels

IS - 5

ER -

Mineralocorticoids modulate the expression of the β-3 subunit of the Na⁺, K⁺-ATPase in the renal collecting duct

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Keywords

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