Optimisation of synthesis of oligosaccharides derived from lactulose (fructosyl-galacto-oligosaccharides) with β-galactosidases of different origin

Cecilia Guerrero, Carlos Vera, Andrés Illanes

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Batch synthesis of fructosyl-galacto-oligosaccharides from lactulose was performed with commercial β-galactosidase preparations from Aspergillus oryzae, Kluyveromyces lactis and Bacillus circulans. The enzyme from A. oryzae produced the highest yield and specific productivity of synthesis, being selected for further studies. Optimization of fructosyl-galacto-oligosaccharides synthesis was carried out using response surface methodology, considering temperature and initial sugar concentration as variables and yield and specific productivity as response parameters. Maximum yield of 0.41 g g-1 fructosyl-galacto-oligosaccharides was obtained at 70 °C and 60% w/w lactulose concentration, while maximum specific productivity of 1.2 g h -1 mg-1 was obtained at 70 °C and 40% w/w lactulose concentration.

Original languageEnglish
Pages (from-to)2225-2232
Number of pages8
JournalFood Chemistry
Volume138
Issue number4
DOIs
StatePublished - 15 Jun 2013
Externally publishedYes

Keywords

  • Fructosyl-galacto- oligosaccharides
  • Galacto-oligosaccharides
  • Lactulose
  • Prebiotic
  • Transgalactosylation
  • β-Galactosidase

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