Optimization of secoisolariciresinol diglucoside extraction from flaxseed (Linum usitatissimum L.) and isolation by a simple HPLC-UV method

CLAUDIA VIVIANA FUENTEALBA CARRASCO, Fernando Figuerola, Ana Mariá Estévez, Adrian González-Munõz, Ociel Munõz

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8 Scopus citations

Abstract

This research was conducted to develop and optimize a methodology to quantify the content of secoisolariciresinol diglucoside (SDG), the main vegetable lignan present in flaxseed. However, at the time of this work, commercial SDG was not available for standard use. Therefore, an effective method to obtain purified SDG from flaxseed was developed using chromatographic techniques. The SDG obtained was of approximately 97% purity. The methanol extraction time, alkali concentrations, and reaction temperature were optimized through a response surface methodology (RSM), and the optimal conditions established for the extractions were 47°C, 58 mmol L-1 sodium methoxide, and 24 h. This methodology was then applied to study the variation of SDG content in flaxseed from different cultivars. The SDG content in different varieties of flaxseed ranged from 10.8 to 17.9 mg g-1 in defatted flaxseed flour and from 6.0 to 10.9 mg g-1 in whole flaxseed.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalCYTA - Journal of Food
Volume13
Issue number2
DOIs
StatePublished - 3 Apr 2015

Keywords

  • Flaxseed
  • lignan
  • RSM
  • secoisolariciresinol diglucoside

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