Metodología optimizada para la extracción de ácidos fenólicos libres y ligados de la accesión de maíz (Zea mays L.) chileno Cristalino

Translated title of the contribution: Optimized methodology for the extraction of free and bound phenolic acids from Chilean Cristalino corn (Zea mays L.) accession

Claudia Fuentealba, Ana M. Quesille-Villalobos, Adrian González-Muñoz, Jorge Saavedra Torrico, Kalidas Shetty, Lena Gálvez Ranilla

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The aim of this study was to optimize the extraction of free and bound phenolic acids from Chilean Cristalino corn by response surface methodology based on the total phenolic contents (TPCs) and the 2,2-diphenyl-1-picrylhydrazyl scavenging-linked antioxidant capacity (AC) as response variables. Central Composite 22 + axial points experimental designs were applied. The best extraction conditions for the free and bound phenolic fraction were acetone 69% in water for 63 min and a hydrolysis with 3 M NaOH for 90 min, respectively. Under these conditions, TPCs in free and bound forms were 59.9 ± 0.7 and 172.9 ± 1.1 mg gallic acid equivalents/100 g, respectively. Further, AC found in free and bound fractions was 186 ± 3 and 694.5 ± 3.3 µmol Trolox equivalents/100 g, respectively. The experimental and predicted values of TPC and AC were similar in case of free phenolic fraction indicating that the model was adequate and reproducible. Major phenolic acids were found in the bound fraction and were ferulic and p-coumaric acids.

Translated title of the contributionOptimized methodology for the extraction of free and bound phenolic acids from Chilean Cristalino corn (Zea mays L.) accession
Original languageSpanish
Pages (from-to)91-98
Number of pages8
JournalCYTA - Journal of Food
Volume15
Issue number1
DOIs
StatePublished - 2 Jan 2017
Externally publishedYes

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