Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission

M. Frómeta; G. Moreno; J. Ricardo; Y. Arias; M. Muramatsu; L. F. Gomes; G. Palácios; F. Palácios; H. Velázquez; JOSE LUIS VALIN RIVERA; L. Ramirez Q

doi:10.1016/j.optcom.2016.11.065

Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission

M. Frómeta, G. Moreno, J. Ricardo, Y. Arias, M. Muramatsu, L. F. Gomes, G. Palácios, F. Palácios, H. Velázquez, JOSE LUIS VALIN RIVERA, L. Ramirez Q

SCHOOL OF MECHANICAL ENGINEERING

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5 Scopus citations

Abstract

In this paper the integral refractive index of a microscopic sample was directly measured by applying Digital Holographic Microscopy (DHM) capturing transmission and reflection holograms simultaneously, of the same sample's region, using Mach-Zehnder and Michelson micro interferometers for transmission and reflection holograms capture and modeling the 3D sample in a medium of known refractive index n_m. The system was calibrated using standard polystyrene sphere immersed in water with known diameter and refractive index, and the method was applied for erythrocyte integral refractive index determination. The results are in accordance with predicted, the measurements error of the order of ±0.005 in absolute values.

Original language	English
Pages (from-to)	252-256
Number of pages	5
Journal	Optics Communications
Volume	387
DOIs	https://doi.org/10.1016/j.optcom.2016.11.065
State	Published - 15 Mar 2017

Keywords

Digital Holographic Microscopy (DHM)
Digital reconstruction
Erythrocyte
Refractive index

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Frómeta, M., Moreno, G., Ricardo, J., Arias, Y., Muramatsu, M., Gomes, L. F., Palácios, G., Palácios, F., Velázquez, H., VALIN RIVERA, JOSE. LUIS., & Ramirez Q, L. (2017). Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission. Optics Communications, 387, 252-256. https://doi.org/10.1016/j.optcom.2016.11.065

Frómeta, M. ; Moreno, G. ; Ricardo, J. ; Arias, Y. ; Muramatsu, M. ; Gomes, L. F. ; Palácios, G. ; Palácios, F. ; Velázquez, H. ; VALIN RIVERA, JOSE LUIS ; Ramirez Q, L. / Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission. In: Optics Communications. 2017 ; Vol. 387. pp. 252-256.

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abstract = "In this paper the integral refractive index of a microscopic sample was directly measured by applying Digital Holographic Microscopy (DHM) capturing transmission and reflection holograms simultaneously, of the same sample's region, using Mach-Zehnder and Michelson micro interferometers for transmission and reflection holograms capture and modeling the 3D sample in a medium of known refractive index nm. The system was calibrated using standard polystyrene sphere immersed in water with known diameter and refractive index, and the method was applied for erythrocyte integral refractive index determination. The results are in accordance with predicted, the measurements error of the order of ±0.005 in absolute values.",

keywords = "Digital Holographic Microscopy (DHM), Digital reconstruction, Erythrocyte, Refractive index",

author = "M. Fr{\'o}meta and G. Moreno and J. Ricardo and Y. Arias and M. Muramatsu and Gomes, {L. F.} and G. Pal{\'a}cios and F. Pal{\'a}cios and H. Vel{\'a}zquez and {VALIN RIVERA}, {JOSE LUIS} and {Ramirez Q}, L.",

year = "2017",

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doi = "10.1016/j.optcom.2016.11.065",

language = "English",

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Frómeta, M, Moreno, G, Ricardo, J, Arias, Y, Muramatsu, M, Gomes, LF, Palácios, G, Palácios, F, Velázquez, H, VALIN RIVERA, JOSELUIS & Ramirez Q, L 2017, 'Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission', Optics Communications, vol. 387, pp. 252-256. https://doi.org/10.1016/j.optcom.2016.11.065

Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission. / Frómeta, M.; Moreno, G.; Ricardo, J.; Arias, Y.; Muramatsu, M.; Gomes, L. F.; Palácios, G.; Palácios, F.; Velázquez, H.; VALIN RIVERA, JOSE LUIS; Ramirez Q, L.

In: Optics Communications, Vol. 387, 15.03.2017, p. 252-256.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission

AU - Frómeta, M.

AU - Moreno, G.

AU - Ricardo, J.

AU - Arias, Y.

AU - Muramatsu, M.

AU - Gomes, L. F.

AU - Palácios, G.

AU - Palácios, F.

AU - Velázquez, H.

AU - VALIN RIVERA, JOSE LUIS

AU - Ramirez Q, L.

PY - 2017/3/15

Y1 - 2017/3/15

N2 - In this paper the integral refractive index of a microscopic sample was directly measured by applying Digital Holographic Microscopy (DHM) capturing transmission and reflection holograms simultaneously, of the same sample's region, using Mach-Zehnder and Michelson micro interferometers for transmission and reflection holograms capture and modeling the 3D sample in a medium of known refractive index nm. The system was calibrated using standard polystyrene sphere immersed in water with known diameter and refractive index, and the method was applied for erythrocyte integral refractive index determination. The results are in accordance with predicted, the measurements error of the order of ±0.005 in absolute values.

AB - In this paper the integral refractive index of a microscopic sample was directly measured by applying Digital Holographic Microscopy (DHM) capturing transmission and reflection holograms simultaneously, of the same sample's region, using Mach-Zehnder and Michelson micro interferometers for transmission and reflection holograms capture and modeling the 3D sample in a medium of known refractive index nm. The system was calibrated using standard polystyrene sphere immersed in water with known diameter and refractive index, and the method was applied for erythrocyte integral refractive index determination. The results are in accordance with predicted, the measurements error of the order of ±0.005 in absolute values.

KW - Digital Holographic Microscopy (DHM)

KW - Digital reconstruction

KW - Erythrocyte

KW - Refractive index

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EP - 256

JO - Optics Communications

JF - Optics Communications

SN - 0030-4018

ER -

Optimized setup for integral refractive index direct determination applying digital holographic microscopy by reflection and transmission

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Keywords

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