Optimizing conditions for successful plant regeneration from cryopreserved castanea sativa shoot tips

LORENA VERONICA JORQUERA MARTINEZ, Nieves Vidal, Conchi Sánchez, Ana M. Vieitez

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

European chestnut is currently being ravaged by ink and blight diseases. Cryopreservation can constitute a complementary approach to field collections for longterm conservation of germplasm. A two-step vitrification methodology (LS-PVS2) was successful in cryopreserving micropropagated Castanea sativa shoot apices so that they could be recovered for plant regeneration. In order to optimize shoot performance after cryopreservation different parameters, such as explant type, duration of PVS2 treatment, sucrose content in preculture medium, loading pre-treatment, and sequence of media used after thawing, were studied.

Original languageEnglish
Title of host publicationIII International Chestnut Congress
PublisherInternational Society for Horticultural Science
Pages511-518
Number of pages8
ISBN (Print)9789066051003
DOIs
StatePublished - 1 Jan 2005

Publication series

NameActa Horticulturae
Volume693
ISSN (Print)0567-7572

Keywords

  • Chestnut
  • Germplasm storage
  • In vitro
  • Shoot apices
  • Vitrification

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