Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering

Janeth I. Galarza; Javier A. Gimpel; Verónica Rojas; Bertha O. Arredondo-Vega; Vitalia Henríquez

doi:10.1016/j.algal.2018.02.024

Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering

Janeth I. Galarza, Javier A. Gimpel, Verónica Rojas, Bertha O. Arredondo-Vega, Vitalia Henríquez

INSTITUTE OF BIOLOGY

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

The carotenoid biosynthesis pathway of Haematococcus pluvialis has been genetically modified to overproduce astaxanthin, a red pigment of high commercial value. The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. Astaxanthin accumulated up to 67% higher in transformed strains than in wild-type upon induction with high light intensity and nitrogen depletion. This is the first report of plastid transformation of a microalgae with its endogenous pds nuclear gene.

Original language	English
Pages (from-to)	291-297
Number of pages	7
Journal	Algal Research
Volume	31
DOIs	https://doi.org/10.1016/j.algal.2018.02.024
State	Published - Apr 2018

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title = "Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering",

abstract = "The carotenoid biosynthesis pathway of Haematococcus pluvialis has been genetically modified to overproduce astaxanthin, a red pigment of high commercial value. The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. Astaxanthin accumulated up to 67% higher in transformed strains than in wild-type upon induction with high light intensity and nitrogen depletion. This is the first report of plastid transformation of a microalgae with its endogenous pds nuclear gene.",

author = "Galarza, {Janeth I.} and Gimpel, {Javier A.} and Ver{\'o}nica Rojas and Arredondo-Vega, {Bertha O.} and Vitalia Henr{\'i}quez",

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doi = "10.1016/j.algal.2018.02.024",

language = "English",

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journal = "Algal Research",

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T1 - Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering

AU - Galarza, Janeth I.

AU - Gimpel, Javier A.

AU - Rojas, Verónica

AU - Arredondo-Vega, Bertha O.

AU - Henríquez, Vitalia

PY - 2018/4

Y1 - 2018/4

N2 - The carotenoid biosynthesis pathway of Haematococcus pluvialis has been genetically modified to overproduce astaxanthin, a red pigment of high commercial value. The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. Astaxanthin accumulated up to 67% higher in transformed strains than in wild-type upon induction with high light intensity and nitrogen depletion. This is the first report of plastid transformation of a microalgae with its endogenous pds nuclear gene.

AB - The carotenoid biosynthesis pathway of Haematococcus pluvialis has been genetically modified to overproduce astaxanthin, a red pigment of high commercial value. The endogenous phytoene desaturase coding sequence (pds) has been codon optimized and overexpressed in the chloroplast of H. pluvialis under the control of the psbA promoter/UTR. After biolistic transformation and selection, PDS mRNA and protein were detected in transformants. Astaxanthin accumulated up to 67% higher in transformed strains than in wild-type upon induction with high light intensity and nitrogen depletion. This is the first report of plastid transformation of a microalgae with its endogenous pds nuclear gene.

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DO - 10.1016/j.algal.2018.02.024

M3 - Article

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SN - 2211-9264

VL - 31

SP - 291

EP - 297

JO - Algal Research

JF - Algal Research

ER -

Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering

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