Parameters for the Evaluation of Immobilized Enzymes Under Process Conditions

Andrés Illanes; Lorena Wilson

doi:10.1007/978-1-0716-0215-7_3

Parameters for the Evaluation of Immobilized Enzymes Under Process Conditions

Andrés Illanes, Lorena Wilson

SCHOOL OF BIOCHEMICAL ENGINEERING

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

11 Scopus citations

Abstract

The characterization of immobilized enzymes allows the evaluation of the immobilization process itself and also the projection of the immobilized enzyme performance under process operation conditions. Based on such characterization, strategies for support functionalization and enzyme immobilization into the activated support can be selected, determining the best conditions for conducting such steps in view of the intended use of the biocatalyst, establishing a linkage between biocatalyst production and biocatalyst use. The determination of the catalytic potential of the immobilized enzyme under operational conditions is a priceless parameter that takes into account both activity and stability, including the effect of both mass transfer limitations (diffusional restrictions) and intrinsic enzyme inactivation upon the immobilization process.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	65-81
Number of pages	17
DOIs	https://doi.org/10.1007/978-1-0716-0215-7_3
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2100
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Catalytic potential
Diffusional restrictions
Enzyme inactivation
Immobilization yield
Inactivation mechanism

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10.1007/978-1-0716-0215-7_3

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AU - Wilson, Lorena

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AB - The characterization of immobilized enzymes allows the evaluation of the immobilization process itself and also the projection of the immobilized enzyme performance under process operation conditions. Based on such characterization, strategies for support functionalization and enzyme immobilization into the activated support can be selected, determining the best conditions for conducting such steps in view of the intended use of the biocatalyst, establishing a linkage between biocatalyst production and biocatalyst use. The determination of the catalytic potential of the immobilized enzyme under operational conditions is a priceless parameter that takes into account both activity and stability, including the effect of both mass transfer limitations (diffusional restrictions) and intrinsic enzyme inactivation upon the immobilization process.

KW - Catalytic potential

KW - Diffusional restrictions

KW - Enzyme inactivation

KW - Immobilization yield

KW - Inactivation mechanism

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AN - SCOPUS:85077890109

T3 - Methods in Molecular Biology

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EP - 81

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Parameters for the Evaluation of Immobilized Enzymes Under Process Conditions

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