Parameters for the Evaluation of Immobilized Enzymes Under Process Conditions

Andrés Illanes, Lorena Wilson

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

11 Scopus citations


The characterization of immobilized enzymes allows the evaluation of the immobilization process itself and also the projection of the immobilized enzyme performance under process operation conditions. Based on such characterization, strategies for support functionalization and enzyme immobilization into the activated support can be selected, determining the best conditions for conducting such steps in view of the intended use of the biocatalyst, establishing a linkage between biocatalyst production and biocatalyst use. The determination of the catalytic potential of the immobilized enzyme under operational conditions is a priceless parameter that takes into account both activity and stability, including the effect of both mass transfer limitations (diffusional restrictions) and intrinsic enzyme inactivation upon the immobilization process.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Catalytic potential
  • Diffusional restrictions
  • Enzyme inactivation
  • Immobilization yield
  • Inactivation mechanism


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