Performance of an enzymatic extract in Botrycoccus braunii cell wall disruption

Gustavo Ciudad, Olga Rubilar, Laura Azócar, Claudio Toro, Mara Cea, Álvaro Torres, Alejandra Ribera, Rodrigo Navia

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Microalgae can produce and contain lipids, proteins and carbohydrates, which can be extracted and marketed as potential novel added-value bio-products. However, microalgae cell wall disruption is one of the most important challenges involved while processing this type of biomass. In this context, white-rot fungi, responsible for the biodegradation of lignin present in wood due to non-specific extracellular enzymes, could be applied for promoting microalgae cell wall degradation. Therefore, the aim of this study was to evaluate the use of an enzymatic extract produced by the white-rot fungi Anthracophyllum discolor as a biotechnological tool for Botryococcus braunii cell wall disruption. The fungus was inoculated in wheat grains and manganese peroxidase (MnP) activity was monitored while obtaining the enzymatic extract. Then, cell wall disruption trials with different MnP activity were evaluated by the biochemical methane potential (BMP). In relation to cell wall disruption, it was observed that the optimal value was obtained with enzymatic concentration of 1000U/L with a BMP of 521mL CH4/g VS. Under these conditions almost 90% of biomass biodegradability was observed, increasing in 62% compared to the microalgae without treatment. Therefore, the results indicate that enzymes secreted by A. discolor promoted the attack of the different cell wall components finally weakening it. Therefore, the application of this treatment could be a promissory biotechnological approach to decrease the energetic input required for the cell wall disruption step.

Original languageEnglish
Pages (from-to)75-80
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume117
Issue number1
DOIs
StatePublished - Jan 2014
Externally publishedYes

Keywords

  • Anthracophyllum discolor
  • Biochemical methane potential
  • Cell wall disruption
  • Ligninolytic enzymes
  • Microalgae

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