Permeability changes induced by polylysines in rat spermatids

Ramon A. Jorquera; Julio Berrios; Jorge Sans; Cecilia Vergara; Dale J. Benos; Juan G. Reyes

doi:10.1016/S0248-4900(02)01198-X

Permeability changes induced by polylysines in rat spermatids

Ramon A. Jorquera, Julio Berrios, Jorge Sans, Cecilia Vergara, Dale J. Benos, Juan G. Reyes

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

High molecular weight (HMW, >15 kDa) but not low molecular weight (LMW, <15 kDa) polylysines (PLs) bound and induced permeability changes in rat spermatid plasma membranes, estimated by Mn²⁺ quenching of intracellular indo-1 fluorescence (K_1/2 = 3.3 ± 0.5 μg/ml) and Co²⁺ quenching of intracellular calcein. The pharmacology of the Mn²⁺ entry pathway activated by HMW PL does not suggest that Ca²⁺ channels are involved in this phenomenon. Concentrations of HMW PL that induced divalent ion entry did not induce the entry of ethidium bromide, suggesting that HMW PL first bound and perturbed the plasma membrane structure inducing a non-specific increase in membrane permeability. High concentrations of HMW PL induced cell lysis (K_1/2 = 23 μg/ml). The binding of HMW PL, initially homogenous on the cell surface, subsequently progressed to a segregated pattern resembling a clustering phenomenon.

Original language	English
Pages (from-to)	233-241
Number of pages	9
Journal	Biology of the Cell
Volume	94
Issue number	4-5
DOIs	https://doi.org/10.1016/S0248-4900(02)01198-X
State	Published - Sep 2002

Keywords

Cationic peptides
Spermatogenic cells

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title = "Permeability changes induced by polylysines in rat spermatids",

abstract = "High molecular weight (HMW, >15 kDa) but not low molecular weight (LMW, <15 kDa) polylysines (PLs) bound and induced permeability changes in rat spermatid plasma membranes, estimated by Mn2+ quenching of intracellular indo-1 fluorescence (K1/2 = 3.3 ± 0.5 μg/ml) and Co2+ quenching of intracellular calcein. The pharmacology of the Mn2+ entry pathway activated by HMW PL does not suggest that Ca2+ channels are involved in this phenomenon. Concentrations of HMW PL that induced divalent ion entry did not induce the entry of ethidium bromide, suggesting that HMW PL first bound and perturbed the plasma membrane structure inducing a non-specific increase in membrane permeability. High concentrations of HMW PL induced cell lysis (K1/2 = 23 μg/ml). The binding of HMW PL, initially homogenous on the cell surface, subsequently progressed to a segregated pattern resembling a clustering phenomenon.",

keywords = "Cationic peptides, Spermatogenic cells",

author = "Jorquera, {Ramon A.} and Julio Berrios and Jorge Sans and Cecilia Vergara and Benos, {Dale J.} and Reyes, {Juan G.}",

note = "Funding Information: We thank Dr. P. Sotomayor and collaborators for allowing us the use of their spectroscopy facilities and Estefan{\'i}a Herrera for excellent technical assistance. Rodrigo Rivera performed the experiments of chondroitinase and heparitinase treatments of the cells. This work was supported by grants from FONDECYT 1990689 and DGIP/UCV.",

year = "2002",

month = sep,

doi = "10.1016/S0248-4900(02)01198-X",

language = "English",

volume = "94",

pages = "233--241",

journal = "Biology of the Cell",

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publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Permeability changes induced by polylysines in rat spermatids

AU - Jorquera, Ramon A.

AU - Berrios, Julio

AU - Sans, Jorge

AU - Vergara, Cecilia

AU - Benos, Dale J.

AU - Reyes, Juan G.

N1 - Funding Information: We thank Dr. P. Sotomayor and collaborators for allowing us the use of their spectroscopy facilities and Estefanía Herrera for excellent technical assistance. Rodrigo Rivera performed the experiments of chondroitinase and heparitinase treatments of the cells. This work was supported by grants from FONDECYT 1990689 and DGIP/UCV.

PY - 2002/9

Y1 - 2002/9

N2 - High molecular weight (HMW, >15 kDa) but not low molecular weight (LMW, <15 kDa) polylysines (PLs) bound and induced permeability changes in rat spermatid plasma membranes, estimated by Mn2+ quenching of intracellular indo-1 fluorescence (K1/2 = 3.3 ± 0.5 μg/ml) and Co2+ quenching of intracellular calcein. The pharmacology of the Mn2+ entry pathway activated by HMW PL does not suggest that Ca2+ channels are involved in this phenomenon. Concentrations of HMW PL that induced divalent ion entry did not induce the entry of ethidium bromide, suggesting that HMW PL first bound and perturbed the plasma membrane structure inducing a non-specific increase in membrane permeability. High concentrations of HMW PL induced cell lysis (K1/2 = 23 μg/ml). The binding of HMW PL, initially homogenous on the cell surface, subsequently progressed to a segregated pattern resembling a clustering phenomenon.

AB - High molecular weight (HMW, >15 kDa) but not low molecular weight (LMW, <15 kDa) polylysines (PLs) bound and induced permeability changes in rat spermatid plasma membranes, estimated by Mn2+ quenching of intracellular indo-1 fluorescence (K1/2 = 3.3 ± 0.5 μg/ml) and Co2+ quenching of intracellular calcein. The pharmacology of the Mn2+ entry pathway activated by HMW PL does not suggest that Ca2+ channels are involved in this phenomenon. Concentrations of HMW PL that induced divalent ion entry did not induce the entry of ethidium bromide, suggesting that HMW PL first bound and perturbed the plasma membrane structure inducing a non-specific increase in membrane permeability. High concentrations of HMW PL induced cell lysis (K1/2 = 23 μg/ml). The binding of HMW PL, initially homogenous on the cell surface, subsequently progressed to a segregated pattern resembling a clustering phenomenon.

KW - Cationic peptides

KW - Spermatogenic cells

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U2 - 10.1016/S0248-4900(02)01198-X

DO - 10.1016/S0248-4900(02)01198-X

M3 - Article

C2 - 12489692

AN - SCOPUS:0036758798

SN - 0248-4900

VL - 94

SP - 233

EP - 241

JO - Biology of the Cell

JF - Biology of the Cell

IS - 4-5

ER -

Permeability changes induced by polylysines in rat spermatids

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