TY - JOUR
T1 - Phenolic profiles of andean mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers
T2 - Identification by HPLC-DAD and evaluation of their antioxidant activity
AU - Chirinos, Rosana
AU - Campos, David
AU - Costa, Nora
AU - Arbizu, Carlos
AU - Pedreschi, Romina
AU - Larondelle, Yvan
N1 - Funding Information:
The authors thank the International Potato Center (Lima, Peru) for providing the mashua genotypes. This research was supported by the CUI project of the Belgian Coopération Universitaire au Développement (CUD, Belgium) and by the Consejo Nacional de Ciencia y Tecnología (CONCYTEC, Peru).
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Qualitative high performance liquid chromatography with diode array detection (HPLC-DAD) was performed to characterize non-anthocyanin phenolic compounds in three different coloured mashua genotypes. The ORAC antioxidant activity contribution in the tubers related to the type of phenolic compounds present was also evaluated. Phenolic compounds were analysed by separating them into four main fractions: fraction I obtained by means of a liquid-liquid partition with ethyl acetate and fractions II, III and IV obtained by elution on a Sephadex LH-20 column. Fraction I revealed the presence of gallic acid, gallocatechin, procyanidin B2 and epigallocatechin. Other phenolic compounds such as hydroxycinnamic and hydroxybenzoic acid derivatives, rutin and/or myricetin derivatives were also present in fraction I. Fraction II was mainly composed of epicatechin, hydroxycinnamic and hydroxybenzoic acid derivatives. Fraction III presented mainly anthocyanins for the purple coloured mashua tubers and rutin, hydroxycinnamic acid and hydroxybenzoic acid derivatives for the yellow coloured genotype. Fraction IV was composed of proanthocyanidins. Alkaline and acid hydrolysis of the different fractions revealed the presence of gallocatechin, epicatechin, p-coumaric acid, o-coumaric acid, cinnamic acid, protocatechuic acid, rutin and quercetin as the main phenolic moieties present. The proanthocyanidin fractions were the major contributors to the ORAC antioxidant activity of the mashua tubers for two of the three genotypes (34.7-39.2%). The results obtained in the present study confirm that mashua tubers constitute a promising source of antioxidant phenolics and could potentially be considered as a functional food with beneficial health effects.
AB - Qualitative high performance liquid chromatography with diode array detection (HPLC-DAD) was performed to characterize non-anthocyanin phenolic compounds in three different coloured mashua genotypes. The ORAC antioxidant activity contribution in the tubers related to the type of phenolic compounds present was also evaluated. Phenolic compounds were analysed by separating them into four main fractions: fraction I obtained by means of a liquid-liquid partition with ethyl acetate and fractions II, III and IV obtained by elution on a Sephadex LH-20 column. Fraction I revealed the presence of gallic acid, gallocatechin, procyanidin B2 and epigallocatechin. Other phenolic compounds such as hydroxycinnamic and hydroxybenzoic acid derivatives, rutin and/or myricetin derivatives were also present in fraction I. Fraction II was mainly composed of epicatechin, hydroxycinnamic and hydroxybenzoic acid derivatives. Fraction III presented mainly anthocyanins for the purple coloured mashua tubers and rutin, hydroxycinnamic acid and hydroxybenzoic acid derivatives for the yellow coloured genotype. Fraction IV was composed of proanthocyanidins. Alkaline and acid hydrolysis of the different fractions revealed the presence of gallocatechin, epicatechin, p-coumaric acid, o-coumaric acid, cinnamic acid, protocatechuic acid, rutin and quercetin as the main phenolic moieties present. The proanthocyanidin fractions were the major contributors to the ORAC antioxidant activity of the mashua tubers for two of the three genotypes (34.7-39.2%). The results obtained in the present study confirm that mashua tubers constitute a promising source of antioxidant phenolics and could potentially be considered as a functional food with beneficial health effects.
KW - Antioxidant properties
KW - HPLC-DAD
KW - Liquid-liquid partition
KW - Mashua
KW - Phenolic profiles
KW - Proanthocyanidins
KW - Sephadex LH-20 chromatography
KW - Tropaeolum tuberosum Ruíz & Pavón
UR - http://www.scopus.com/inward/record.url?scp=34548509030&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2007.07.024
DO - 10.1016/j.foodchem.2007.07.024
M3 - Article
AN - SCOPUS:34548509030
SN - 0308-8146
VL - 106
SP - 1285
EP - 1298
JO - Food Chemistry
JF - Food Chemistry
IS - 3
ER -