Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes

LUIS ALBERTO MERCADO VIANCO, Asunción Cao, Ramiro Barcia, Juan Ignacio Ramos-Martinez

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalMolecular and Cellular Biochemistry
Volume250
Issue number1-2
DOIs
StatePublished - 1 Aug 2003

Keywords

  • Hemocytes
  • Immunity
  • Mussel
  • Mytilus
  • PKC
  • Translocation

Fingerprint Dive into the research topics of 'Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes'. Together they form a unique fingerprint.

Cite this