Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes

Luís Mercado; Asunción Cao; Ramiro Barcia; Juan Ignacio Ramos-Martinez

doi:10.1023/A:1024981814050

Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes

Luís Mercado, Asunción Cao, Ramiro Barcia, Juan Ignacio Ramos-Martinez

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.

Original language	English
Pages (from-to)	41-45
Number of pages	5
Journal	Molecular and Cellular Biochemistry
Volume	250
Issue number	1-2
DOIs	https://doi.org/10.1023/A:1024981814050
State	Published - Aug 2003
Externally published	Yes

Keywords

Hemocytes
Immunity
Mussel
Mytilus
PKC
Translocation

Access to Document

10.1023/A:1024981814050

Cite this

@article{fd5418fc3d6b402da84bd26d8350c12b,

title = "Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes",

abstract = "Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.",

keywords = "Hemocytes, Immunity, Mussel, Mytilus, PKC, Translocation",

author = "Lu{\'i}s Mercado and Asunci{\'o}n Cao and Ramiro Barcia and Ramos-Martinez, {Juan Ignacio}",

note = "Funding Information: This work was supported by the grant PGIDT MAR26102PR from the Autonomous Government of Galicia (Spain). The authors wish to thank Mar{\'i}a Mosquera for excellent technical assistance.",

year = "2003",

month = aug,

doi = "10.1023/A:1024981814050",

language = "English",

volume = "250",

pages = "41--45",

journal = "Molecular and Cellular Biochemistry",

issn = "0300-8177",

publisher = "Springer Netherlands",

number = "1-2",

}

TY - JOUR

T1 - Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes

AU - Mercado, Luís

AU - Cao, Asunción

AU - Barcia, Ramiro

AU - Ramos-Martinez, Juan Ignacio

N1 - Funding Information: This work was supported by the grant PGIDT MAR26102PR from the Autonomous Government of Galicia (Spain). The authors wish to thank María Mosquera for excellent technical assistance.

PY - 2003/8

Y1 - 2003/8

N2 - Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.

AB - Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.

KW - Hemocytes

KW - Immunity

KW - Mussel

KW - Mytilus

KW - PKC

KW - Translocation

UR - http://www.scopus.com/inward/record.url?scp=0042386235&partnerID=8YFLogxK

U2 - 10.1023/A:1024981814050

DO - 10.1023/A:1024981814050

M3 - Article

C2 - 12962141

AN - SCOPUS:0042386235

SN - 0300-8177

VL - 250

SP - 41

EP - 45

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 1-2

ER -

Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this