Polymerase chain reaction (PCR) detection of B cell clonality in Sjögren's syndrome patients: A diagnostic tool of clonal expansion

LEDA MARCELA GUZMAN MALUENDA, D. Castillo, S. O. Aguilera

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Sjögren's syndrome (SS) is an autoimmune disease characterized by clonal B cell attack of the exocrine glands and dysregulated expression of B cell-activating factor (BAFF). Based upon the current data of increased rates of lymphoid malignancy, as non-Hodgkin's lymphoma (NHL) is associated with SS, we propose the detection of clonal rearrangements of immunoglobulin heavy chain (IgH) gene in those patients as a predictor of malignant clonal expansion. To test our proposal, we examined the IgH clonal rearrangements in SS patients (60) and healthy control subjects (42) having chronic non-specific sialadenitis, to determine the presence of clonal B cells in minor labial salivary glands (MSG) of SS patients. Clonal B cell expansion was assessed by two polymerase chain reaction (PCR) assays: (i) semi-nested PCR, against sequences encoding framework regions FR3, FR2 and FR1c of the variable chain IgH gene in B cells present in the MSG infiltrate; and (ii) the PCR-enzyme-linked immunosorbent assay (ELISA) technique, against the major and minor breakpoint regions of the Bcl-2 oncogene coupled with a variable segment of the IgH to assess the Bcl-2/JH translocation. When FR3, FR2 and FR1c primers were employed, we detected B cell monoclonality in 87% of the SS patients and 19% of the control subjects. The association between inflammation severity of the MSG pattern and the presence of B cell clonality was found to be statistically significant (P < 0·01). We concluded that the presence of B cell clonality in MSG can be used as a index of an altered microenvironment favouring the development of lymphoma in SS patients.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalClinical and Experimental Immunology
Volume161
Issue number1
DOIs
StatePublished - Jul 2010
Externally publishedYes

Keywords

  • B cell clonality
  • Chronic sialadenitis
  • Immunoglobulin heavy chain gene
  • PCR

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