A new method for microscopic object analysis is suggested in this research. We consider the diffraction theory combined with the image formation process, this combination constitutes the groundwork of many optic transformation processes that it has made possible to establish several modern applications of the Fourier optics processing and Digital Holographic Microscopy (DHM). Based on these optical applications we consider to study microscopic objects with regular forms starting from its Fraunhofer diffraction pattern obtained with DHM. The first results correspond to objects with regular forms and randomly distributed in the space, the second result corresponds to objects with regular forms and periodically distributed in the space. The objects' parameters can be determined with the diffraction pattern manipulation in a simple and accurate way. The biological and materials sciences can be beneficed with this research.