TY - JOUR
T1 - Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides
AU - Rojas, Bárbara
AU - Suárez-Vega, Felipe
AU - Saez-Aguayo, Susana
AU - Olmedo, Patricio
AU - Zepeda, Baltasar
AU - Delgado-Rioseco, Joaquín
AU - Defilippi, Bruno G.
AU - Pedreschi, Romina
AU - Meneses, Claudio
AU - Pérez-Donoso, Alonso G.
AU - Campos-Vargas, Reinaldo
N1 - Funding Information:
This work was supported by ANID CONICYT/FONDECYT/REGULAR/1200260 (to R.C.-V.) and 1201467 (to S.S.-A.).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - The use of plant growth regulators (PGRs) is widespread in commercial table grape vine-yards. The synthetic cytokinin CPPU is a PGR that is extensively used to obtain higher quality grapes. However, the effect of CPPU on berry firmness is not clear. The current study investigated the effects of pre-anthesis applications (BBCH15 and BBCH55 stages) of CPPU on ‘Thompson Seedless’ berry firmness at harvest through a combination of cytological, morphological, and biochemical analyses. Ovaries in CPPU-treated plants presented morphological changes related to cell division and cell wall modification at the anthesis stage (BBCH65). Moreover, immunofluorescence analysis with monoclonal antibodies 2F4 and LM15 against pectin and xyloglucan demonstrated that CPPU treatment resulted in cell wall modifications at anthesis. These early changes have major repercussions regarding the hemicellulose and pectin cell wall composition of mature fruits, and are associated with increased calcium content and a higher berry firmness at harvest.
AB - The use of plant growth regulators (PGRs) is widespread in commercial table grape vine-yards. The synthetic cytokinin CPPU is a PGR that is extensively used to obtain higher quality grapes. However, the effect of CPPU on berry firmness is not clear. The current study investigated the effects of pre-anthesis applications (BBCH15 and BBCH55 stages) of CPPU on ‘Thompson Seedless’ berry firmness at harvest through a combination of cytological, morphological, and biochemical analyses. Ovaries in CPPU-treated plants presented morphological changes related to cell division and cell wall modification at the anthesis stage (BBCH65). Moreover, immunofluorescence analysis with monoclonal antibodies 2F4 and LM15 against pectin and xyloglucan demonstrated that CPPU treatment resulted in cell wall modifications at anthesis. These early changes have major repercussions regarding the hemicellulose and pectin cell wall composition of mature fruits, and are associated with increased calcium content and a higher berry firmness at harvest.
KW - Cell wall
KW - CPPU
KW - Cytokinin
KW - Pre-anthesis
KW - Thompson Seedless
KW - Vitis vinifera
UR - http://www.scopus.com/inward/record.url?scp=85120305284&partnerID=8YFLogxK
U2 - 10.3390/plants10122642
DO - 10.3390/plants10122642
M3 - Article
AN - SCOPUS:85120305284
VL - 10
JO - Plants
JF - Plants
SN - 2223-7747
IS - 12
M1 - 2642
ER -