Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides

Bárbara Rojas; Felipe Suárez-Vega; Susana Saez-Aguayo; Patricio Olmedo; Baltasar Zepeda; Joaquín Delgado-Rioseco; Bruno G. Defilippi; Romina Pedreschi; Claudio Meneses; Alonso G. Pérez-Donoso; Reinaldo Campos-Vargas

doi:10.3390/plants10122642

Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides

Bárbara Rojas, Felipe Suárez-Vega, Susana Saez-Aguayo, Patricio Olmedo, Baltasar Zepeda, Joaquín Delgado-Rioseco, Bruno G. Defilippi, Romina Pedreschi, Claudio Meneses, Alonso G. Pérez-Donoso, Reinaldo Campos-Vargas

SCHOOL OF AGRONOMY

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The use of plant growth regulators (PGRs) is widespread in commercial table grape vine-yards. The synthetic cytokinin CPPU is a PGR that is extensively used to obtain higher quality grapes. However, the effect of CPPU on berry firmness is not clear. The current study investigated the effects of pre-anthesis applications (BBCH15 and BBCH55 stages) of CPPU on ‘Thompson Seedless’ berry firmness at harvest through a combination of cytological, morphological, and biochemical analyses. Ovaries in CPPU-treated plants presented morphological changes related to cell division and cell wall modification at the anthesis stage (BBCH65). Moreover, immunofluorescence analysis with monoclonal antibodies 2F4 and LM15 against pectin and xyloglucan demonstrated that CPPU treatment resulted in cell wall modifications at anthesis. These early changes have major repercussions regarding the hemicellulose and pectin cell wall composition of mature fruits, and are associated with increased calcium content and a higher berry firmness at harvest.

Original language	English
Article number	2642
Journal	Plants
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/plants10122642
State	Published - Dec 2021

Keywords

CPPU
Cell wall
Cytokinin
Pre-anthesis
Thompson Seedless
Vitis vinifera

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10.3390/plants10122642

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title = "Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides",

abstract = "The use of plant growth regulators (PGRs) is widespread in commercial table grape vine-yards. The synthetic cytokinin CPPU is a PGR that is extensively used to obtain higher quality grapes. However, the effect of CPPU on berry firmness is not clear. The current study investigated the effects of pre-anthesis applications (BBCH15 and BBCH55 stages) of CPPU on {\textquoteleft}Thompson Seedless{\textquoteright} berry firmness at harvest through a combination of cytological, morphological, and biochemical analyses. Ovaries in CPPU-treated plants presented morphological changes related to cell division and cell wall modification at the anthesis stage (BBCH65). Moreover, immunofluorescence analysis with monoclonal antibodies 2F4 and LM15 against pectin and xyloglucan demonstrated that CPPU treatment resulted in cell wall modifications at anthesis. These early changes have major repercussions regarding the hemicellulose and pectin cell wall composition of mature fruits, and are associated with increased calcium content and a higher berry firmness at harvest.",

keywords = "CPPU, Cell wall, Cytokinin, Pre-anthesis, Thompson Seedless, Vitis vinifera",

author = "B{\'a}rbara Rojas and Felipe Su{\'a}rez-Vega and Susana Saez-Aguayo and Patricio Olmedo and Baltasar Zepeda and Joaqu{\'i}n Delgado-Rioseco and Defilippi, {Bruno G.} and Romina Pedreschi and Claudio Meneses and P{\'e}rez-Donoso, {Alonso G.} and Reinaldo Campos-Vargas",

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year = "2021",

month = dec,

doi = "10.3390/plants10122642",

language = "English",

volume = "10",

journal = "Plants",

issn = "2223-7747",

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T1 - Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides

AU - Rojas, Bárbara

AU - Suárez-Vega, Felipe

AU - Saez-Aguayo, Susana

AU - Olmedo, Patricio

AU - Zepeda, Baltasar

AU - Delgado-Rioseco, Joaquín

AU - Defilippi, Bruno G.

AU - Pedreschi, Romina

AU - Meneses, Claudio

AU - Pérez-Donoso, Alonso G.

AU - Campos-Vargas, Reinaldo

PY - 2021/12

Y1 - 2021/12

N2 - The use of plant growth regulators (PGRs) is widespread in commercial table grape vine-yards. The synthetic cytokinin CPPU is a PGR that is extensively used to obtain higher quality grapes. However, the effect of CPPU on berry firmness is not clear. The current study investigated the effects of pre-anthesis applications (BBCH15 and BBCH55 stages) of CPPU on ‘Thompson Seedless’ berry firmness at harvest through a combination of cytological, morphological, and biochemical analyses. Ovaries in CPPU-treated plants presented morphological changes related to cell division and cell wall modification at the anthesis stage (BBCH65). Moreover, immunofluorescence analysis with monoclonal antibodies 2F4 and LM15 against pectin and xyloglucan demonstrated that CPPU treatment resulted in cell wall modifications at anthesis. These early changes have major repercussions regarding the hemicellulose and pectin cell wall composition of mature fruits, and are associated with increased calcium content and a higher berry firmness at harvest.

AB - The use of plant growth regulators (PGRs) is widespread in commercial table grape vine-yards. The synthetic cytokinin CPPU is a PGR that is extensively used to obtain higher quality grapes. However, the effect of CPPU on berry firmness is not clear. The current study investigated the effects of pre-anthesis applications (BBCH15 and BBCH55 stages) of CPPU on ‘Thompson Seedless’ berry firmness at harvest through a combination of cytological, morphological, and biochemical analyses. Ovaries in CPPU-treated plants presented morphological changes related to cell division and cell wall modification at the anthesis stage (BBCH65). Moreover, immunofluorescence analysis with monoclonal antibodies 2F4 and LM15 against pectin and xyloglucan demonstrated that CPPU treatment resulted in cell wall modifications at anthesis. These early changes have major repercussions regarding the hemicellulose and pectin cell wall composition of mature fruits, and are associated with increased calcium content and a higher berry firmness at harvest.

KW - CPPU

KW - Cell wall

KW - Cytokinin

KW - Pre-anthesis

KW - Thompson Seedless

KW - Vitis vinifera

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U2 - 10.3390/plants10122642

DO - 10.3390/plants10122642

M3 - Article

AN - SCOPUS:85120305284

SN - 2223-7747

VL - 10

JO - Plants

JF - Plants

IS - 12

M1 - 2642

ER -

Pre-anthesis cytokinin applications increase table grape berry firmness by modulating cell wall polysaccharides

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Keywords

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