Primary cultures of hemocytes from Mytilus galloprovincialis Lmk.: Expression of IL-2Rα subunit

Asunción Cao; Luis Mercado; Juan Ignacio Ramos-Martinez; Ramiro Barcia

doi:10.1016/S0044-8486(02)00140-0

Primary cultures of hemocytes from Mytilus galloprovincialis Lmk. Expression of IL-2Rα subunit

Asunción Cao, Luis Mercado, Juan Ignacio Ramos-Martinez, Ramiro Barcia

INSTITUTE OF BIOLOGY

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Abstract

Hemocytes from the mollusk Mytilus galloprovincialis Lmk. were maintained for more than 20 days in Leibovitz L-15 medium supplemented with salts and antibiotics. Survival of cells cultured in foetal calf serum (FCS)-supplemented L-15 medium was similar to that of those cultured in inactivated hemolymph-supplemented L-15 medium. Cell activation by lipopolysaccharide (LPS) induces an increase of the number of α-subunits of the interleukin-2 (IL-2) receptor (IL-2Rα) in the cell membrane. The best response in the expression of IL-2Rα subunit was obtained after 3 days of culture. In cultured cells, as in fresh hemocytes, LPS induced a higher expression of IL-2Rα subunit than that induced by IL-2.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Aquaculture
Volume	216
Issue number	1-4
DOIs	https://doi.org/10.1016/S0044-8486(02)00140-0
State	Published - 10 Feb 2003

Keywords

Cell culture
Flow cytometry
Hemocytes
IL-2
IL-2Rα
Immunity
LPS
Mollusk
Mytilus

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10.1016/S0044-8486(02)00140-0

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title = "Primary cultures of hemocytes from Mytilus galloprovincialis Lmk.: Expression of IL-2Rα subunit",

abstract = "Hemocytes from the mollusk Mytilus galloprovincialis Lmk. were maintained for more than 20 days in Leibovitz L-15 medium supplemented with salts and antibiotics. Survival of cells cultured in foetal calf serum (FCS)-supplemented L-15 medium was similar to that of those cultured in inactivated hemolymph-supplemented L-15 medium. Cell activation by lipopolysaccharide (LPS) induces an increase of the number of α-subunits of the interleukin-2 (IL-2) receptor (IL-2Rα) in the cell membrane. The best response in the expression of IL-2Rα subunit was obtained after 3 days of culture. In cultured cells, as in fresh hemocytes, LPS induced a higher expression of IL-2Rα subunit than that induced by IL-2.",

keywords = "Cell culture, Flow cytometry, Hemocytes, IL-2, IL-2Rα, Immunity, LPS, Mollusk, Mytilus",

author = "Asunci{\'o}n Cao and Luis Mercado and Ramos-Martinez, {Juan Ignacio} and Ramiro Barcia",

note = "Funding Information: This work was supported by the Grants (XUGA 26100 PB96 and PGIDTOOMAR26102PR) from the autonomous Government of Galicia (Spain). We thank Maria Mosquera for excellent technical assistance.",

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T1 - Primary cultures of hemocytes from Mytilus galloprovincialis Lmk.

T2 - Expression of IL-2Rα subunit

AU - Cao, Asunción

AU - Mercado, Luis

AU - Ramos-Martinez, Juan Ignacio

AU - Barcia, Ramiro

N1 - Funding Information: This work was supported by the Grants (XUGA 26100 PB96 and PGIDTOOMAR26102PR) from the autonomous Government of Galicia (Spain). We thank Maria Mosquera for excellent technical assistance.

PY - 2003/2/10

Y1 - 2003/2/10

N2 - Hemocytes from the mollusk Mytilus galloprovincialis Lmk. were maintained for more than 20 days in Leibovitz L-15 medium supplemented with salts and antibiotics. Survival of cells cultured in foetal calf serum (FCS)-supplemented L-15 medium was similar to that of those cultured in inactivated hemolymph-supplemented L-15 medium. Cell activation by lipopolysaccharide (LPS) induces an increase of the number of α-subunits of the interleukin-2 (IL-2) receptor (IL-2Rα) in the cell membrane. The best response in the expression of IL-2Rα subunit was obtained after 3 days of culture. In cultured cells, as in fresh hemocytes, LPS induced a higher expression of IL-2Rα subunit than that induced by IL-2.

AB - Hemocytes from the mollusk Mytilus galloprovincialis Lmk. were maintained for more than 20 days in Leibovitz L-15 medium supplemented with salts and antibiotics. Survival of cells cultured in foetal calf serum (FCS)-supplemented L-15 medium was similar to that of those cultured in inactivated hemolymph-supplemented L-15 medium. Cell activation by lipopolysaccharide (LPS) induces an increase of the number of α-subunits of the interleukin-2 (IL-2) receptor (IL-2Rα) in the cell membrane. The best response in the expression of IL-2Rα subunit was obtained after 3 days of culture. In cultured cells, as in fresh hemocytes, LPS induced a higher expression of IL-2Rα subunit than that induced by IL-2.

KW - Cell culture

KW - Flow cytometry

KW - Hemocytes

KW - IL-2

KW - IL-2Rα

KW - Immunity

KW - LPS

KW - Mollusk

KW - Mytilus

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U2 - 10.1016/S0044-8486(02)00140-0

DO - 10.1016/S0044-8486(02)00140-0

M3 - Article

AN - SCOPUS:0037429219

SN - 0044-8486

VL - 216

SP - 1

EP - 8

JO - Aquaculture

JF - Aquaculture

IS - 1-4

ER -

Primary cultures of hemocytes from Mytilus galloprovincialis Lmk. Expression of IL-2Rα subunit

Abstract

Keywords

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