Primary cultures of hemocytes from Mytilus galloprovincialis Lmk. Expression of IL-2Rα subunit

Asunción Cao, LUIS ALBERTO MERCADO VIANCO, Juan Ignacio Ramos-Martinez, Ramiro Barcia

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70 Scopus citations

Abstract

Hemocytes from the mollusk Mytilus galloprovincialis Lmk. were maintained for more than 20 days in Leibovitz L-15 medium supplemented with salts and antibiotics. Survival of cells cultured in foetal calf serum (FCS)-supplemented L-15 medium was similar to that of those cultured in inactivated hemolymph-supplemented L-15 medium. Cell activation by lipopolysaccharide (LPS) induces an increase of the number of α-subunits of the interleukin-2 (IL-2) receptor (IL-2Rα) in the cell membrane. The best response in the expression of IL-2Rα subunit was obtained after 3 days of culture. In cultured cells, as in fresh hemocytes, LPS induced a higher expression of IL-2Rα subunit than that induced by IL-2.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalAquaculture
Volume216
Issue number1-4
DOIs
StatePublished - 10 Feb 2003

Keywords

  • Cell culture
  • Flow cytometry
  • Hemocytes
  • IL-2
  • IL-2Rα
  • Immunity
  • LPS
  • Mollusk
  • Mytilus

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