TY - JOUR
T1 - Protective effect of an antimicrobial peptide from Mytilus edulis chilensis expressed in Nicotiana tabacum L.
AU - Arenas, Gloria
AU - Marshall, Sergio H.
AU - Espinoza, Valeria
AU - Ramírez, Ingrid
AU - Peña-Cortés, Hugo
PY - 2006/4/15
Y1 - 2006/4/15
N2 - A "defensin-like" antibacterial peptide from Mytilus edulis chilensis, was sub-cloned into a binary vector for expression in plant tissues. The resulting new clone was electroporated into A. tumefaciens to transform tobacco plants. The presence of the construct in transgenic tobacco lines was demonstrated through RT-PCR, Northern and Western blots. Transformed positive plants were selected and grown for challenging. Tobacco leaves were infiltrated with Pseudomonassyringae pv. syringae and visual lesions determined at different times post-exposure. Of seven plants exposed, four gave variable protection up to seven days post-infection while one of them appears to be fully protected. These results suggest that defensin-like antimicrobial peptides from molluscs are a good source to provide resistance of tobacco plants to Pseudomonassyringae pv. syringae.
AB - A "defensin-like" antibacterial peptide from Mytilus edulis chilensis, was sub-cloned into a binary vector for expression in plant tissues. The resulting new clone was electroporated into A. tumefaciens to transform tobacco plants. The presence of the construct in transgenic tobacco lines was demonstrated through RT-PCR, Northern and Western blots. Transformed positive plants were selected and grown for challenging. Tobacco leaves were infiltrated with Pseudomonassyringae pv. syringae and visual lesions determined at different times post-exposure. Of seven plants exposed, four gave variable protection up to seven days post-infection while one of them appears to be fully protected. These results suggest that defensin-like antimicrobial peptides from molluscs are a good source to provide resistance of tobacco plants to Pseudomonassyringae pv. syringae.
KW - Antibacterial peptide
KW - Disease resistance
KW - In vivo expression
KW - Nicotiana tabacum L.
KW - Pseudomonassyringae pv. syringae
KW - Transgenic tobacco plants
UR - http://www.scopus.com/inward/record.url?scp=33646145092&partnerID=8YFLogxK
U2 - 10.2225/vol9-issue2-fulltext-2
DO - 10.2225/vol9-issue2-fulltext-2
M3 - Article
AN - SCOPUS:33646145092
VL - 9
SP - 144
EP - 151
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
SN - 0717-3458
IS - 2
ER -