Purity of mushroom tyrosinase as a biocatalyst for biomateriai synthesis affects the stability of therapeutic peptides

M. Racheva, OSCAR ENRIQUE ROMERO ORMAZABAL, K. K. Julich-Gruner, A. S. Ulrich, C. Wischke, A. Lendlein

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

The formation of injectable implants in the presence of cells or solutes has previously been conceptualized to be based on the selectivity of bioorthogonal chemical reactions. As an alternative approach, hydrogel network synthesis by enzymatic reactions with a typically high inherent substrate specificity and low toxicity have been repeatedly proposed, e.g. using commercial mushroom tyrosinase (MTyr), which specifically catalyzes phenol oxidation. In this study, it should be explored whether MTyr is compatible with therapeutic peptides that may be delivered from such hydrogels in the future. Based on the specificity of MTyr to phenol residues, no modification of peptides lacking the amino acid tyrosine would be expected. One example of such peptides is gramicidin S (GS), a potent antimicrobial peptide. However, when GS was incubated with commercial MTyr, peptide degradation occurred as observed by HPLC analysis. Several fragments of the peptide were detected by MALDI-TOF. Contamination of MTyr with peptidases was proven as the source of undesired peptide cleavage, which needs to be considered when preparing enzymatically crosslinked hydrogels for biomedical applications.

Original languageEnglish
Title of host publicationMultifunctional Polymeric and Hybrid Materials
EditorsTao Xie, Nicola Tirelli, Andreas Lendlein, Robert A. Weiss, Andreas Lendlein
PublisherMaterials Research Society
Pages85-90
Number of pages6
ISBN (Electronic)9781605116952
DOIs
StatePublished - 1 Jan 2015
Event2014 MRS Fall Meeting - Boston, United States
Duration: 30 Nov 20145 Dec 2014

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1718
ISSN (Print)0272-9172

Conference

Conference2014 MRS Fall Meeting
CountryUnited States
CityBoston
Period30/11/145/12/14

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