Quantification of collagen fibers in canine uteri treated with medroxyprogesterone acetate

Paulo Salinas, Maria A. Miglino, Mariano del Sol

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Collagen plays essential roles in remodeling uterine tissue during decidualization, implantation, pregnancy and involution. To understand whether the progestational agent medroxyprogesterone acetate (MPA) can modify the organization and deposit of collagen in the uteri of normal bitches (Canis Tlupus familiaris), we assessed uterine tissues by histochemistry. Uteri were grouped as: nulliparous (n=11), multiparous (n=11) and treated with MPA (n=11; nulliparous with two treatments; 5mg/kg; i.m.). The amount, location and birefringence of interstitial collagen types I and III in the fold and base of the endometrial stroma and the myometrial muscular layers were studied on sections stained with Picrosirius Red by polarized light microscopy and evaluated by ANOVA. No differences were observed in the endometrium. In the myometrium, differences were observed in collagen type I between MPA-treated and nulliparous uteri vs. multiparous (p<0.05), and differences in collagen type III between nulliparous and multiparous uteri vs. MPA-treated (p=0.0001). In conclusion, two doses of MPA had no significant effect on the investigated collagens in the extracellular matrix.

Original languageEnglish
Pages (from-to)1221-1226
Number of pages6
JournalPesquisa Veterinaria Brasileira
Volume36
Issue number12
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • Canis lupus familiaris
  • Collagen
  • Medroxyprogesterone acetate
  • Picrosirius red
  • Uterus

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