Rapid Enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals

Karla Cautivo; Tony Schountz; Mariana Acuña-Retamar; Marcela Ferrés; Fernando Torres-Pérez

doi:10.3390/v6052028

Rapid Enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals

Karla Cautivo, Tony Schountz, Mariana Acuña-Retamar, Marcela Ferrés, Fernando Torres-Pérez

INSTITUTE OF BIOLOGY

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.

Original language	English
Pages (from-to)	2028-2037
Number of pages	10
Journal	Viruses
Volume	6
Issue number	5
DOIs	https://doi.org/10.3390/v6052028
State	Published - 6 May 2014

Keywords

Andes virus
Chile
ELISA
Hantavirus
Strip immunoblot assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v6052028

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abstract = "We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.",

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AU - Cautivo, Karla

AU - Schountz, Tony

AU - Acuña-Retamar, Mariana

AU - Ferrés, Marcela

AU - Torres-Pérez, Fernando

PY - 2014/5/6

Y1 - 2014/5/6

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AB - We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.

KW - Andes virus

KW - Chile

KW - ELISA

KW - Hantavirus

KW - Strip immunoblot assay

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Rapid Enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals

Abstract

Keywords

UN SDGs

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