Rapid Enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals

Karla Cautivo, Tony Schountz, Mariana Acuña-Retamar, Marcela Ferrés, FERNANDO LEON TORRES PEREZ

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.

Original languageEnglish
Pages (from-to)2028-2037
Number of pages10
JournalViruses
Volume6
Issue number5
DOIs
StatePublished - 6 May 2014

Keywords

  • Andes virus
  • Chile
  • ELISA
  • Hantavirus
  • Strip immunoblot assay

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