TY - JOUR
T1 - Raw galacto-oligosaccharide purification by consecutive lactose hydrolysis and selective bioconversion
AU - Santibáñez, Luciana
AU - Guerrero, Cecilia
AU - Illanes, Andrés
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/12
Y1 - 2017/12
N2 - Galacto-oligosaccharide (GOS) yields obtained by enzymatic transgalactosylation with β-galactosidases are rather low, so purification of the raw GOS is a major issue. In the quest for improving GOS purification, an additional step of lactose hydrolysis is proposed, using immobilised β-galactosidase and permeabilised cells expressing β-galactosidase activity as biocatalysts. To select the best operational conditions for lactose hydrolysis in raw GOS employing both biocatalysts different enzyme:substrate (lactose) mass ratios were evaluated. Bioconversion of the lactose-hydrolysed raw GOS obtained with the two biocatalyst forms were compared, and biocatalyst reuse was evaluated. Lactose hydrolysis of raw GOS allowed the removal of around 70% of the lactose content and increased the relative GOS content by 20%. Incorporation of the lactose hydrolysis step led to a purity and recovery of GOS of 96–97% and 92–96%, respectively, depending on the biocatalyst used.
AB - Galacto-oligosaccharide (GOS) yields obtained by enzymatic transgalactosylation with β-galactosidases are rather low, so purification of the raw GOS is a major issue. In the quest for improving GOS purification, an additional step of lactose hydrolysis is proposed, using immobilised β-galactosidase and permeabilised cells expressing β-galactosidase activity as biocatalysts. To select the best operational conditions for lactose hydrolysis in raw GOS employing both biocatalysts different enzyme:substrate (lactose) mass ratios were evaluated. Bioconversion of the lactose-hydrolysed raw GOS obtained with the two biocatalyst forms were compared, and biocatalyst reuse was evaluated. Lactose hydrolysis of raw GOS allowed the removal of around 70% of the lactose content and increased the relative GOS content by 20%. Incorporation of the lactose hydrolysis step led to a purity and recovery of GOS of 96–97% and 92–96%, respectively, depending on the biocatalyst used.
UR - http://www.scopus.com/inward/record.url?scp=85028448537&partnerID=8YFLogxK
U2 - 10.1016/j.idairyj.2017.07.008
DO - 10.1016/j.idairyj.2017.07.008
M3 - Article
AN - SCOPUS:85028448537
SN - 0958-6946
VL - 75
SP - 91
EP - 100
JO - International Dairy Journal
JF - International Dairy Journal
ER -