During the last 30. years the scope of biocatalysis has been expanding due to the advances in several technological fields. Diverse techniques as structural enzyme improvement (. e.g. protein engineering, direct evolution), engineering approaches (. e.g. ionic liquids, supercritical fluids) and physical stabilization (. e.g. immobilization, CLEAS) have been developed, which in combination are powerful tools to improve biotransformation and to synthesize new products. In the present work, recent advances in biocatalysis are reviewed.
- Biocatalysis in organic solvents
- Direct evolution
- Rational design