Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature

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Abstract

Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l-1 at 144 h of cultivation) and tPA specific production rate (47 ng 10-6 cell h-1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was observed, suggesting that tPA production rate would be directly controlled by the growth rate.

Original languageEnglish
Pages (from-to)1493-1497
Number of pages5
JournalBiotechnology Letters
Volume31
Issue number10
DOIs
StatePublished - Aug 2009
Externally publishedYes

Keywords

  • CHO cells
  • Glutamate
  • Low temperature
  • Mannose
  • Specific growth rate
  • tPA

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