Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature

Julio Berrios; Alvaro Díaz-Barrera; Consuelo Bazán; Claudia Altamirano

doi:10.1007/s10529-009-0050-1

Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature

Julio Berrios, Alvaro Díaz-Barrera, Consuelo Bazán, Claudia Altamirano

SCHOOL OF BIOCHEMICAL ENGINEERING

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20 Scopus citations

Abstract

Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l^-1 at 144 h of cultivation) and tPA specific production rate (47 ng 10^-6 cell h^-1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was observed, suggesting that tPA production rate would be directly controlled by the growth rate.

Original language	English
Pages (from-to)	1493-1497
Number of pages	5
Journal	Biotechnology Letters
Volume	31
Issue number	10
DOIs	https://doi.org/10.1007/s10529-009-0050-1
State	Published - Aug 2009

Keywords

CHO cells
Glutamate
Low temperature
Mannose
Specific growth rate
tPA

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10.1007/s10529-009-0050-1

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title = "Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature",

abstract = "Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l-1 at 144 h of cultivation) and tPA specific production rate (47 ng 10-6 cell h-1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was observed, suggesting that tPA production rate would be directly controlled by the growth rate.",

keywords = "CHO cells, Glutamate, Low temperature, Mannose, Specific growth rate, tPA",

author = "Julio Berrios and Alvaro D{\'i}az-Barrera and Consuelo Baz{\'a}n and Claudia Altamirano",

note = "Funding Information: Acknowledgment Authors acknowledge Projects FOND-ECYT 1070337 and DI-PUCV 203.758 for financial support, and Pharmacia (Uppsala, Sweden) for providing the cell line. Technical assistance of Ver{\'o}nica Figueroa and Catalina Morales is also acknowledged.",

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doi = "10.1007/s10529-009-0050-1",

language = "English",

volume = "31",

pages = "1493--1497",

journal = "Biotechnology Letters",

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publisher = "Springer Netherlands",

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T1 - Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature

AU - Berrios, Julio

AU - Díaz-Barrera, Alvaro

AU - Bazán, Consuelo

AU - Altamirano, Claudia

N1 - Funding Information: Acknowledgment Authors acknowledge Projects FOND-ECYT 1070337 and DI-PUCV 203.758 for financial support, and Pharmacia (Uppsala, Sweden) for providing the cell line. Technical assistance of Verónica Figueroa and Catalina Morales is also acknowledged.

PY - 2009/8

Y1 - 2009/8

N2 - Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l-1 at 144 h of cultivation) and tPA specific production rate (47 ng 10-6 cell h-1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was observed, suggesting that tPA production rate would be directly controlled by the growth rate.

AB - Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l-1 at 144 h of cultivation) and tPA specific production rate (47 ng 10-6 cell h-1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was observed, suggesting that tPA production rate would be directly controlled by the growth rate.

KW - CHO cells

KW - Glutamate

KW - Low temperature

KW - Mannose

KW - Specific growth rate

KW - tPA

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U2 - 10.1007/s10529-009-0050-1

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AN - SCOPUS:70350103519

SN - 0141-5492

VL - 31

SP - 1493

EP - 1497

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 10

ER -

Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature

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Keywords

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