Selection and validation of reliable housekeeping genes to evaluate Piscirickettsia salmonis gene expression

Patricio Flores-Herrera, Oscar Arredondo-Zelada, SERGIO HERNAN MARSHALL GONZALEZ, FERNANDO ANDRES GOMEZ CARMONA

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Piscirickettsia salmonis is a highly aggressive facultative intracellular bacterium that challenges the sustainability of Chilean salmon production. Due to the limited knowledge of its biology, there is a need to identify key molecular markers that could help define the pathogenic potential of this bacterium. We think a model system should be implemented that efficiently evaluates the expression of putative bacterial markers by using validated, stable, and highly specific housekeeping genes to properly select target genes, which could lead to identifying those responsible for infection and disease induction in naturally infected fish. Here, we selected a set of validated reference or housekeeping genes for RT-qPCR expression analyses of P. salmonis under different growth and stress conditions, including an in vitro infection kinetic. After a thorough screening, we selected sdhA as the most reliable housekeeping gene able to represent stable and highly specific host reference genes for RT-qPCR-driven P. salmonis analysis.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalInfection, Genetics and Evolution
Volume63
DOIs
StatePublished - 1 Sep 2018

Keywords

  • Housekeeping gene
  • Pisciricketssia
  • RT-qPCR analysys

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