TY - JOUR
T1 - Senescent cultures of human dermal fibroblasts modified phenotype when immobilized in fibrin polymer
AU - Acevedo, Cristian A.
AU - Brown, Donald I.
AU - Young, Manuel E.
AU - Reyes, Juan G.
N1 - Funding Information:
The authors wish to thank CONICYT from Chile for financial support through a FONDEF Grant (No. DO2I1009) and Doctoral Scholarship for C. A. (No. D-21050588).
PY - 2009/9/1
Y1 - 2009/9/1
N2 - One of the limitations in tissue engineering is the restricted ability to expand the number of cells, because somatic cells can duplicate a limited number of times before they lose the ability to divide, leading to a senescent state. Here we report that the interaction of senescent fibroblasts with fibrin polymer can modify the senescent phenotype and partially restore the ability of growth-arrested cells to continue replicating. Primary human dermal fibroblasts were grown to >90% SA/β-Gal (senescence associated β-galactosidase) . The senescent cells were immobilized in fibrin-polymers by mixing fibrinogen and thrombin solutions. Immobilized senescent cell cultures grew, however, their growth arrested after 24 h of immobilization. The percentage of cells with a positive reaction at SA/β-Gal did not decrease significantly after immobilization, but the intensity of the stain decreased. The glycolytic activity in immobilized senescent fibroblast was re-established at pre-senescent levels. In conclusion, fibrin induces changes in the phenotype of senescent human fibroblasts. This simple procedure could complement available tissue-engineering techniques to increase the amount of biomass seeded on a fibrin scaffold, which could be beyond senescence.
AB - One of the limitations in tissue engineering is the restricted ability to expand the number of cells, because somatic cells can duplicate a limited number of times before they lose the ability to divide, leading to a senescent state. Here we report that the interaction of senescent fibroblasts with fibrin polymer can modify the senescent phenotype and partially restore the ability of growth-arrested cells to continue replicating. Primary human dermal fibroblasts were grown to >90% SA/β-Gal (senescence associated β-galactosidase) . The senescent cells were immobilized in fibrin-polymers by mixing fibrinogen and thrombin solutions. Immobilized senescent cell cultures grew, however, their growth arrested after 24 h of immobilization. The percentage of cells with a positive reaction at SA/β-Gal did not decrease significantly after immobilization, but the intensity of the stain decreased. The glycolytic activity in immobilized senescent fibroblast was re-established at pre-senescent levels. In conclusion, fibrin induces changes in the phenotype of senescent human fibroblasts. This simple procedure could complement available tissue-engineering techniques to increase the amount of biomass seeded on a fibrin scaffold, which could be beyond senescence.
KW - Fibrin
KW - Human fibroblasts
KW - Immobilization
KW - Senescence
UR - http://www.scopus.com/inward/record.url?scp=71749106297&partnerID=8YFLogxK
U2 - 10.1163/156856208X394418
DO - 10.1163/156856208X394418
M3 - Article
C2 - 19793448
AN - SCOPUS:71749106297
VL - 20
SP - 1929
EP - 1942
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 13
ER -