TY - JOUR
T1 - Sequence Polymorphism and Expression Variability of Crassostrea gigas Immune Related Genes Discriminate Two Oyster Lines Contrasted in Term of Resistance to Summer Mortalities
AU - Schmitt, Paulina
AU - Santini, Adrien
AU - Vergnes, Agnès
AU - Degremont, Lionel
AU - de Lorgeril, Julien
N1 - Funding Information:
We are very grateful to Delphine Destoumieux-Garzón for helpful discussion, Timothy Green for the English corrections of the manuscript, Arthur Escalas for his advice in statistical analysis and Marc Leroy for technical assistance. Oyster used in this work were produced and maintained in controlled conditions through technical facilities of the Station de Bouin–Laboratoire Génétique et Pathologie, and the Aquaculture Platform located at Ifremer Palaeovas. Data used in this work were partly produced through molecular genetic analysis technical facilities of the "Plate-forme génotypage séquençage CeMEB (Centre méditerranéen de l’Environnement et de la biodiversité)" And the ‘"Plate-forme qPHD UM2/Montpellier GenomiX". P. Schmitt was supported by a doctoral funding from Program Becas Chile-CONICYT.
PY - 2013/9/26
Y1 - 2013/9/26
N2 - Summer mortalities of Crassostrea gigas are a major concern in oyster aquaculture. They are the result of a complex interaction between the host, pathogens and environmental factors. Oyster genetics have been identified as an essential determinant of oyster susceptibility to summer mortalities. As the capability of oysters to circumvent diseases depends in part on their immune defenses, we aimed to analyze the gene expression and sequence polymorphism of 42 immune related genes in two oyster lines selected for their "High" (H) and "Low" (L) survival to summer mortalities. Results showed that the variability of gene expression and the sequence polymorphism acting on particular genes could enable the discrimination between H and L oyster lines. Besides, a higher sequence polymorphism was observed on the L line affecting 11 of the 42 analyzed genes. By analyzing gene expression, sequence polymorphism and gene copy number of two antimicrobial peptide families (Cg-Defs and Cg-Prp), and an antimicrobial protein (Cg-BPI) on individual oysters, we showed that gene expression and/or sequence polymorphism could also discriminate H and L oyster lines. Finally, we observed a positive correlation between the gene expression and the gene copy number of antimicrobials and that sequence polymorphism could be encoded in the genome. Overall, this study gives new insights in the relationship between oyster immunity and divergent phenotypes, and discusses the potential implication of antimicrobial diversity in oyster survival to summer mortalities.
AB - Summer mortalities of Crassostrea gigas are a major concern in oyster aquaculture. They are the result of a complex interaction between the host, pathogens and environmental factors. Oyster genetics have been identified as an essential determinant of oyster susceptibility to summer mortalities. As the capability of oysters to circumvent diseases depends in part on their immune defenses, we aimed to analyze the gene expression and sequence polymorphism of 42 immune related genes in two oyster lines selected for their "High" (H) and "Low" (L) survival to summer mortalities. Results showed that the variability of gene expression and the sequence polymorphism acting on particular genes could enable the discrimination between H and L oyster lines. Besides, a higher sequence polymorphism was observed on the L line affecting 11 of the 42 analyzed genes. By analyzing gene expression, sequence polymorphism and gene copy number of two antimicrobial peptide families (Cg-Defs and Cg-Prp), and an antimicrobial protein (Cg-BPI) on individual oysters, we showed that gene expression and/or sequence polymorphism could also discriminate H and L oyster lines. Finally, we observed a positive correlation between the gene expression and the gene copy number of antimicrobials and that sequence polymorphism could be encoded in the genome. Overall, this study gives new insights in the relationship between oyster immunity and divergent phenotypes, and discusses the potential implication of antimicrobial diversity in oyster survival to summer mortalities.
UR - http://www.scopus.com/inward/record.url?scp=84884641644&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0075900
DO - 10.1371/journal.pone.0075900
M3 - Article
C2 - 24086661
AN - SCOPUS:84884641644
SN - 1932-6203
VL - 8
JO - PLoS ONE
JF - PLoS ONE
IS - 9
M1 - e75900
ER -