TY - JOUR
T1 - Solvent inhalation (toluene and n-hexane) during the brain growth spurt impairs the maturation of frontal, parietal and occipital cerebrocortical neurons in rats
AU - Pascual, R.
AU - Aedo, Luz
AU - Meneses, Juan Carlos
AU - Vergara, Daniela
AU - Reyes, Álvaro
AU - Bustamante, Carlos
PY - 2010/10
Y1 - 2010/10
N2 - Solvent abuse during pregnancy may cause " fetal solvent syndrome" , which is characterized by mild brain atrophy and associated with behavioral, cognitive, and emotional abnormalities. The present study investigated whether solvent inhalation during the preweaning period (P2-P21) alters the morphological maturation of frontal, parietal, and occipital cortical neurons. Twelve hours after delivery (postnatal day 0, P0), litters were cross-fostered, culled to 8 pups/dam and housed together with a dam in standard laboratory cages. Litters were randomly assigned to the " air-only" group (n=64, 8 litters) and to the " solvent-sniffer" group (n=72, 9 litters). During P2-P21, each animal was exposed daily to either organic solvent vapors (75% toluene and 18% n-hexane, a solvent mixture commonly found in glues and adhesives) or clean air. To determine the impact of early solvent inhalation on cortical neuronal differentiation, brains were stained using the Golgi-Cox-Sholl procedure to quantitatively assess neocortical pyramidal cell dendrogenesis. Preweaning, solvent-exposed animals displayed dramatic impairments in dendritic growth as well as significant reductions in brain weight and size.
AB - Solvent abuse during pregnancy may cause " fetal solvent syndrome" , which is characterized by mild brain atrophy and associated with behavioral, cognitive, and emotional abnormalities. The present study investigated whether solvent inhalation during the preweaning period (P2-P21) alters the morphological maturation of frontal, parietal, and occipital cortical neurons. Twelve hours after delivery (postnatal day 0, P0), litters were cross-fostered, culled to 8 pups/dam and housed together with a dam in standard laboratory cages. Litters were randomly assigned to the " air-only" group (n=64, 8 litters) and to the " solvent-sniffer" group (n=72, 9 litters). During P2-P21, each animal was exposed daily to either organic solvent vapors (75% toluene and 18% n-hexane, a solvent mixture commonly found in glues and adhesives) or clean air. To determine the impact of early solvent inhalation on cortical neuronal differentiation, brains were stained using the Golgi-Cox-Sholl procedure to quantitatively assess neocortical pyramidal cell dendrogenesis. Preweaning, solvent-exposed animals displayed dramatic impairments in dendritic growth as well as significant reductions in brain weight and size.
KW - Brain growth spurt
KW - Cortical neurons
KW - Dendritic development
KW - Solvent inhalation
UR - http://www.scopus.com/inward/record.url?scp=77955422763&partnerID=8YFLogxK
U2 - 10.1016/j.ijdevneu.2010.06.003
DO - 10.1016/j.ijdevneu.2010.06.003
M3 - Article
C2 - 20600790
AN - SCOPUS:77955422763
SN - 0736-5748
VL - 28
SP - 491
EP - 495
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
IS - 6
ER -