TY - JOUR
T1 - Speciation analysis of antimony in marine biota by HPLC-(UV)-HG-AFS
T2 - Extraction procedures and stability of antimony species
AU - De Gregori, Ida
AU - Quiroz, Waldo
AU - Pinochet, Hugo
AU - Pannier, Florence
AU - Potin-Gautier, Martine
N1 - Funding Information:
The authors gratefully acknowledge the financial support of FONDECYT (project 1030897), Pontificia Universidad Católica de Valparaíso (project 125.755) and the Program ECOS-CONICYT (Scientific Cooperation between France and Chile), through the Action C01E010. W. Quiroz also thanks CONICYT and the Government of France for the fellowships granted.
PY - 2007/9/30
Y1 - 2007/9/30
N2 - Speciation analysis of antimony in marine biota is not well documented, and no specific extraction procedure of antimony species from algae and mollusk samples can be found in the literature. This work presents a suitable methodology for the speciation of antimony in marine biota (algae and mollusk samples). The extraction efficiency of total antimony and the stability of Sb(III), Sb(V) and trimethylantimony(V) in different extraction media (water at 25 and 90 °C, methanol, EDTA and citric acid) were evaluated by analyzing the algae Macrosystis integrifolia (0.55 ± 0.04 μg Sb g-1) and the mollusk Mytilus edulis (0.23 ± 0.01 μg Sb g-1). The speciation analysis was performed by anion exchange liquid chromatography (post-column photo-oxidation) and hydride generation atomic fluorescence spectrometry as detection system (HPLC-(UV)-HG-AFS). Results demonstrated that, based on the extraction yield and the stability, EDTA proved to be the best extracting solution for the speciation analysis of antimony in these matrices. The selected procedure was applied to antimony speciation in different algae samples collected from the Chilean coast. Only the inorganic Sb(V) and Sb(III) species were detected in the extracts. In all analyzed algae the sum of total antimony extracted (determined in the extracts after digestion) and the antimony present in the residue was in good agreement with the total antimony concentration determined by HG-AFS. However, in some extracts the sum of antimony species detected was lower than the total extracted, revealing the presence of unknown antimony species, possibly retained on the column or not detected by HPLC-(UV)-HG-AFS. Further work must be carried out to elucidate the identity of these unknown species of antimony.
AB - Speciation analysis of antimony in marine biota is not well documented, and no specific extraction procedure of antimony species from algae and mollusk samples can be found in the literature. This work presents a suitable methodology for the speciation of antimony in marine biota (algae and mollusk samples). The extraction efficiency of total antimony and the stability of Sb(III), Sb(V) and trimethylantimony(V) in different extraction media (water at 25 and 90 °C, methanol, EDTA and citric acid) were evaluated by analyzing the algae Macrosystis integrifolia (0.55 ± 0.04 μg Sb g-1) and the mollusk Mytilus edulis (0.23 ± 0.01 μg Sb g-1). The speciation analysis was performed by anion exchange liquid chromatography (post-column photo-oxidation) and hydride generation atomic fluorescence spectrometry as detection system (HPLC-(UV)-HG-AFS). Results demonstrated that, based on the extraction yield and the stability, EDTA proved to be the best extracting solution for the speciation analysis of antimony in these matrices. The selected procedure was applied to antimony speciation in different algae samples collected from the Chilean coast. Only the inorganic Sb(V) and Sb(III) species were detected in the extracts. In all analyzed algae the sum of total antimony extracted (determined in the extracts after digestion) and the antimony present in the residue was in good agreement with the total antimony concentration determined by HG-AFS. However, in some extracts the sum of antimony species detected was lower than the total extracted, revealing the presence of unknown antimony species, possibly retained on the column or not detected by HPLC-(UV)-HG-AFS. Further work must be carried out to elucidate the identity of these unknown species of antimony.
KW - Algae
KW - Antimony
KW - Extraction
KW - HPLC-HG-AFS
KW - Mollusk
KW - Speciation
UR - http://www.scopus.com/inward/record.url?scp=34548410553&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2007.04.015
DO - 10.1016/j.talanta.2007.04.015
M3 - Article
AN - SCOPUS:34548410553
SN - 0039-9140
VL - 73
SP - 458
EP - 465
JO - Talanta
JF - Talanta
IS - 3
ER -
