Study cases of enzymatic processes

Sonia Barberis, Fanny Guzmán, Andrés Illanes, Josep López-Santín, Lorena Wilson, Gregorio Álvaro, José M. Guisán, Roberto Fernández-Lafuente, César Mateo, Pere Clapés, Juan M. Lema, Gemma Eibes, Carmen López, M. Teresa Moreira, Gumersindo Feijoo

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

11 Scopus citations

Abstract

Peptides are heteropolymers composed by amino acid residues linked by peptidic bonds between the carboxyl group of one amino acid residue and the α-amino group of the next one. The definition is rather vague in terms of chain length, peptides ranging from two residues to a few dozens residues. Its upper limit of molecular mass has been set rather arbitrarily in 6,000 Da. The size of the molecule determines the technology most suitable for its production. Recombinant DNA technology is particularly suitable for the synthesis of large peptides and proteins, as illustrated by the case of insulin and other hormones (Walsh 2005). Chemical synthesis is a viable technology for the production of small and medium size peptides ranging from about 5 to 80 residues (Kimmerlin and Seebach 2005). Enzymatic synthesis is more restricted and has been hardly applied for the synthesis of peptides exceeding 10 residues. Its potential relies on the synthesis of very small peptides and, in fact, most of the cases reported correspond to dipeptides and tripeptides (Kumar and Bhalla 2005). In this sense, the technologies for peptide production are not competitive with each other in most of the cases.

Original languageEnglish
Title of host publicationEnzyme Biocatalysis
Subtitle of host publicationPrinciples and Applications
PublisherSpringer Netherlands
Pages253-378
Number of pages126
ISBN (Print)9781402083600
DOIs
StatePublished - 2008
Externally publishedYes

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