TY - JOUR
T1 - Study of microalgal behaviour in continuous culture using photosynthetic rate curves
T2 - The case of chlorophyll and carotenoid production by Chlorella vulgaris
AU - Soto-Ramírez, Robinson
AU - Tavernini, Luigi
AU - Zúñiga, Héctor
AU - POIRRIER GONZALEZ, PAOLA
AU - CHAMY MAGGI, ROLANDO ARTURO
N1 - Publisher Copyright:
© 2021 John Wiley & Sons Ltd
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - Chlorella vulgaris (C. vulgaris) is a green microalga of commercial importance as a valuable source of chlorophyll, carotenoids and other products. It is known that variations of light and nutrient availability prompt adaptation mechanisms to maximize photosynthesis and growth, but scant research has been conducted in continuous culture. This work approached the study of C. vulgaris grown in continuous culture under nitrogen and light limitation in terms of pigment content and photosynthetic activity. Results showed that light limitation increased the number and size of the photosynthetic apparatus, showing a maximum photosynthetic rate of 0.98 mgO2 g−1biomass min−1. Nitrogen limitation exerted a detrimental effect on biomass concentration and photosynthetic rate, but was more efficient in terms of oxygen evolved per unit mass of chlorophyll than the light-restricted culture. High levels of pigments were obtained in light limited conditions with 79.1 mg/g of total chlorophyll and 14.2 mg/g of carotenoids, being nearly two times higher than values reported in batch culture for C. vulgaris. Also, pigment productivity was about 6 times higher in this work. These are promising results that make the continuous production of pigments a profitable alternative to batch systems. Further research is advisable on the optimization of continuous culture parameters for pigment production.
AB - Chlorella vulgaris (C. vulgaris) is a green microalga of commercial importance as a valuable source of chlorophyll, carotenoids and other products. It is known that variations of light and nutrient availability prompt adaptation mechanisms to maximize photosynthesis and growth, but scant research has been conducted in continuous culture. This work approached the study of C. vulgaris grown in continuous culture under nitrogen and light limitation in terms of pigment content and photosynthetic activity. Results showed that light limitation increased the number and size of the photosynthetic apparatus, showing a maximum photosynthetic rate of 0.98 mgO2 g−1biomass min−1. Nitrogen limitation exerted a detrimental effect on biomass concentration and photosynthetic rate, but was more efficient in terms of oxygen evolved per unit mass of chlorophyll than the light-restricted culture. High levels of pigments were obtained in light limited conditions with 79.1 mg/g of total chlorophyll and 14.2 mg/g of carotenoids, being nearly two times higher than values reported in batch culture for C. vulgaris. Also, pigment productivity was about 6 times higher in this work. These are promising results that make the continuous production of pigments a profitable alternative to batch systems. Further research is advisable on the optimization of continuous culture parameters for pigment production.
KW - carotenoids
KW - chlorella vulgaris
KW - chlorophyll
KW - continuous culture
KW - nutrient limitation
KW - photosynthetic activity
UR - http://www.scopus.com/inward/record.url?scp=85102430447&partnerID=8YFLogxK
U2 - 10.1111/are.15208
DO - 10.1111/are.15208
M3 - Article
AN - SCOPUS:85102430447
JO - Aquaculture Research
JF - Aquaculture Research
SN - 1355-557X
ER -