Superresolution imaging and force characterization of optical tweezers using high-speed cameras

J. P. Staforelli, J. M. Brito, ESTEBAN MAURICIO VERA ROJAS, S. Torres, C. Saavedra

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The advantageous use of high speed cameras have been lately proposed for the direct characterization of optical tweezers in order to replace indirect measurements often performed by quadrant detectors, at the expense of a tradeoff between acquisition speed and resolution. In the proposed approach, subpixel motion data of the trapped particle is retrieved from a high speed low resolution video sequence. Due to the particular nature of the motion, we propose that the obtained motion information is not only useful for providing online characteristics of the optical trap force, but also for tackling the lack of resolution by means of applying superresolution algorithms on the low resolution image sequence. The results show that the proposed approach allows the characterization of the optical tweezers obtaining the real particle motion directly from the image domain, while still providing simultaneous high resolution imaging.

Original languageEnglish
Title of host publicationFrontiers in Optics, FiO 2009
PublisherOptical Society of America (OSA)
ISBN (Print)9781557528780
DOIs
StatePublished - 2009
Externally publishedYes
EventFrontiers in Optics, FiO 2009 - San Jose, CA, United States
Duration: 11 Oct 200915 Oct 2009

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceFrontiers in Optics, FiO 2009
Country/TerritoryUnited States
CitySan Jose, CA
Period11/10/0915/10/09

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