Synthesis of galacto-oligosaccharides at very high lactose concentrations with immobilized β-galactosidases from Aspergillus oryzae

Leopoldo Martín Huerta, Carlos Vera, Cecilia Guerrero, Lorena Wilson, Andrés Illanes

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

β-Galactosidase from Aspergillus oryzae was immobilized by different methods, being the enzyme immobilized in glyoxyl-agarose selected for the synthesis of galacto-oligosaccharides from lactose at high concentrations. The synthesis was optimized with respect to lactose concentration and enzyme to substrate ratio using lactose conversion into galacto-oligosaccharides as objective function. Optimum values were 54.6% (w/v) lactose and 33 IU g -1 lactose and at such conditions conversion was close to 30%, similar to the one obtained with soluble β-galactosidase and comparable with the best reported values for immobilized β-galactosidases. In the sequential batch production of galacto-oligosaccharides, biocatalyst efficiency was increased by 190% with respect to the free enzyme in solution, and 8500 g of galacto-oligosaccharides per gram of enzyme preparation were produced after 10 batches. This amount can be further increased by considering additional batches up to the point of biocatalyst replacement and is significantly higher than the maximum attainable by using the soluble enzyme.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalProcess Biochemistry
Volume46
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

Keywords

  • Aspergillus oryzae
  • Enzyme reactor
  • Galacto-oligosaccharides
  • Immobilized β-galactosidase
  • Prebiotic

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