TY - JOUR
T1 - Synthesis of galacto-oligosaccharides at very high lactose concentrations with immobilized β-galactosidases from Aspergillus oryzae
AU - Huerta, Leopoldo Martín
AU - Vera, Carlos
AU - Guerrero, Cecilia
AU - Wilson, Lorena
AU - Illanes, Andrés
N1 - Funding Information:
This work was financially supported by Chilean Fondecyt Grant 1100050 and Grant 037-112/2008 from the Pontificia Universidad Católica de Valparaíso and conducted within the framework of CYTED-D Enznut and NovelProbio CYTED-D networks. We acknowledge the generous donations of Friesland Foods (Zwolle, The Netherlands), Kochi S.A. (Santiago, Chile), Enzyme Development Corporation (New York, USA) and Resindion S.R.L. (Rome, Italy). We also acknowledge Ms. Rosa Arrieta and Dr. Fanny Guzmán for their analytical assistance.
PY - 2011/1
Y1 - 2011/1
N2 - β-Galactosidase from Aspergillus oryzae was immobilized by different methods, being the enzyme immobilized in glyoxyl-agarose selected for the synthesis of galacto-oligosaccharides from lactose at high concentrations. The synthesis was optimized with respect to lactose concentration and enzyme to substrate ratio using lactose conversion into galacto-oligosaccharides as objective function. Optimum values were 54.6% (w/v) lactose and 33 IU g -1 lactose and at such conditions conversion was close to 30%, similar to the one obtained with soluble β-galactosidase and comparable with the best reported values for immobilized β-galactosidases. In the sequential batch production of galacto-oligosaccharides, biocatalyst efficiency was increased by 190% with respect to the free enzyme in solution, and 8500 g of galacto-oligosaccharides per gram of enzyme preparation were produced after 10 batches. This amount can be further increased by considering additional batches up to the point of biocatalyst replacement and is significantly higher than the maximum attainable by using the soluble enzyme.
AB - β-Galactosidase from Aspergillus oryzae was immobilized by different methods, being the enzyme immobilized in glyoxyl-agarose selected for the synthesis of galacto-oligosaccharides from lactose at high concentrations. The synthesis was optimized with respect to lactose concentration and enzyme to substrate ratio using lactose conversion into galacto-oligosaccharides as objective function. Optimum values were 54.6% (w/v) lactose and 33 IU g -1 lactose and at such conditions conversion was close to 30%, similar to the one obtained with soluble β-galactosidase and comparable with the best reported values for immobilized β-galactosidases. In the sequential batch production of galacto-oligosaccharides, biocatalyst efficiency was increased by 190% with respect to the free enzyme in solution, and 8500 g of galacto-oligosaccharides per gram of enzyme preparation were produced after 10 batches. This amount can be further increased by considering additional batches up to the point of biocatalyst replacement and is significantly higher than the maximum attainable by using the soluble enzyme.
KW - Aspergillus oryzae
KW - Enzyme reactor
KW - Galacto-oligosaccharides
KW - Immobilized β-galactosidase
KW - Prebiotic
UR - http://www.scopus.com/inward/record.url?scp=78650229995&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2010.08.018
DO - 10.1016/j.procbio.2010.08.018
M3 - Article
AN - SCOPUS:78650229995
VL - 46
SP - 245
EP - 252
JO - Process Biochemistry
JF - Process Biochemistry
SN - 1359-5113
IS - 1
ER -