TY - JOUR
T1 - Tea bags for fmoc solid-phase peptide synthesis
T2 - An example of circular economy
AU - Guzmán, Fanny
AU - Gauna, Adriana
AU - Roman, Tanya
AU - Luna, Omar
AU - Álvarez, Claudio
AU - Pareja-Barrueto, Claudia
AU - Mercado, Luis
AU - Albericio, Fernando
AU - Cárdenas, Constanza
N1 - Funding Information:
Funding: This research was funded by grants to F.G. (REDES 180203 and Fondecyt 1210056) from the National Agency for Research and Development (ANID), Chile. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska Curie grant agreement No. 861190 (PAVE). The work of Omar Luna in Spain is funded by PAVE.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/8/2
Y1 - 2021/8/2
N2 - Peptide synthesis is an area with a wide field of application, from biomedicine to nanotech-nology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols gener-ate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemi-cal features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained.
AB - Peptide synthesis is an area with a wide field of application, from biomedicine to nanotech-nology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols gener-ate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemi-cal features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained.
KW - Green chemistry
KW - Reagents’ recycling
KW - Simultaneous synthesis
UR - http://www.scopus.com/inward/record.url?scp=85113401859&partnerID=8YFLogxK
U2 - 10.3390/molecules26165035
DO - 10.3390/molecules26165035
M3 - Article
AN - SCOPUS:85113401859
VL - 26
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 16
M1 - 5035
ER -