Tea bags for fmoc solid-phase peptide synthesis: An example of circular economy

Fanny Guzmán, Adriana Gauna, Tanya Roman, Omar Luna, Claudio Álvarez, Claudia Pareja-Barrueto, Luis Mercado, Fernando Albericio, Constanza Cárdenas

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Peptide synthesis is an area with a wide field of application, from biomedicine to nanotech-nology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols gener-ate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemi-cal features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained.

Original languageEnglish
Article number5035
Issue number16
StatePublished - 2 Aug 2021


  • Green chemistry
  • Reagents’ recycling
  • Simultaneous synthesis


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