TY - JOUR
T1 - Tea bags for fmoc solid-phase peptide synthesis
T2 - An example of circular economy
AU - Guzmán, Fanny
AU - Gauna, Adriana
AU - Roman, Tanya
AU - Luna, Omar
AU - Álvarez, Claudio
AU - Pareja-Barrueto, Claudia
AU - Mercado, Luis
AU - Albericio, Fernando
AU - Cárdenas, Constanza
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/8/2
Y1 - 2021/8/2
N2 - Peptide synthesis is an area with a wide field of application, from biomedicine to nanotech-nology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols gener-ate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemi-cal features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained.
AB - Peptide synthesis is an area with a wide field of application, from biomedicine to nanotech-nology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols gener-ate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included. Fifty-two peptides with wide amino acid composition and seven to twenty amino acid residues in length were synthesized in less than three weeks. A clustering analysis was performed, grouping the peptides by physicochemi-cal features. Although a relationship between the overall yield and the physicochemical features of the sequences was not established, the process showed good performance despite sequence diversity. The recycling system allowed to reduce N, N-dimethylformamide usage by 25–30% and reduce the deprotection reagent usage by 50%. This protocol has been optimized for the simultaneous synthesis of a large number of peptide sequences. Additionally, a reagent recycling system was included in the procedure, which turns the process into a framework of circular economy, without affecting the quality of the products obtained.
KW - Green chemistry
KW - Reagents’ recycling
KW - Simultaneous synthesis
UR - http://www.scopus.com/inward/record.url?scp=85113401859&partnerID=8YFLogxK
U2 - 10.3390/molecules26165035
DO - 10.3390/molecules26165035
M3 - Article
AN - SCOPUS:85113401859
SN - 1420-3049
VL - 26
JO - Molecules
JF - Molecules
IS - 16
M1 - 5035
ER -