Temperature optimization for reactor operation with chitin-immobilized lactase under modulated inactivation

A. Illanes, LORENA EVELYN WILSON SOTO, G. Tomasello

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Temperature effects on all kinetic and inactivation parameters have been determined for chitin immobilized lactase from Kluyveromyces marxianus var. marxianus, and proper temperature functions have been validated. Maximum reaction rate, Michaelis constant referred to lactose, inhibition constant for galactose and inactivation rates increased with temperature. Enzyme inactivation was adequately modelled by a two-stage series mechanism. The effect of galactose and lactose on enzyme inactivation was determined in terms of modulation factors that were positive for galactose and negative for lactose over the whole range of temperature studied. Modulation factors were mild functions of temperature in the first stage and strong functions in the second stage of CIL inactivation where galactose positive modulation factors increase with temperature and lactose negative modulation factors decrease with temperature. Temperature explicit functions for all kinetic and inactivation parameters were incorporated into a scheme to optimize the temperature of operation for a sequential batch reactor with chitin-immobilized lactase, based on an annual cost objective function for reactor operation. Software for temperature optimization was developed creating a friendly interface with user that allows the introduction of variations in all parameters and operational criteria to perform sensitivity analysis. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)270-278
Number of pages9
JournalEnzyme and Microbial Technology
Volume27
Issue number3-5
DOIs
StatePublished - Aug 2000
Externally publishedYes

Keywords

  • Enzyme inactivation
  • Enzyme reactor
  • Immobilized lactase
  • Temperature optimization

Fingerprint Dive into the research topics of 'Temperature optimization for reactor operation with chitin-immobilized lactase under modulated inactivation'. Together they form a unique fingerprint.

Cite this