TY - JOUR
T1 - The medusa of Aurelia coerulea is similar to its polyp in molecular composition and different from the medusa of Stomolophus meleagris in toxicity
AU - Li, Xiaoya
AU - Ma, Xueqi
AU - Chen, Xintong
AU - Wang, Tingfang
AU - Liu, Qing
AU - Wang, Yongfang
AU - Li, Zhezhe
AU - Höfer, Juan
AU - Li, Fangbing
AU - Xiao, Liang
AU - Sun, Xuejun
AU - Mo, Jinggang
N1 - Funding Information:
This work was supported by the International Science and Technology Cooperation Program of Ministry of Science and Technology of China (2019YFE0116800), Future Warfare Medical Protection Technology Research and Development Special General Project of Naval Military Medical University in 2019 (19WLUMS-09), Key Scientific Research Fund Naval Medical Center of PLA , Medical Security Center (18ML01) and the general program of the National Natural Science Foundation of China (81971824). The authors thank Dr. Xuxia Li and Dr. Jishun Yang for language polishing and processing.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/4/30
Y1 - 2022/4/30
N2 - Background: The incidents of Aurelia sp. stinging have recently increased because of a bloom in offshore area. However, their symptoms are much milder than those from another scyphozoan jellyfish, Stomolophus meleagris. Methods: The molecular composition of the medusa and polyp of Aurelia coerulea was analyzed by sequencing the transcriptome and proteome. The toxicity of tentacle extract from A. coerulea medusa (A-TE) and S. meleagris medusa (S-TE) was measured by the survival rates of mice, their blood indexes, and integrity of red blood cells. Results: The medusa and polyp of A. coerulea are similar in molecular composition, while their gene expressions are significantly different at both transcriptome and proteome levels. A-TE displayed no in vitro hemolysis and caused mild damage to the liver, heart and kidney instead of lethality. In contrast, S-TE showed strong hemolytic toxicity, and lethal effect with serious damage to the liver, heart and kidney. The toxin screening in the medusae showed that there were similar toxin categories though the number of toxin species in A. coerulea was larger than that in S. meleagris. Among them, lactotransferrin and venom prothrombin activator were the two predominant protein toxins in the medusae of A. coerulea and S. meleagris, respectively. Conclusions: A. coerulea medusa and polyp have similar molecular compositions, though there are observable morphological differences. The toxicity of A. coerulea medusa is significantly weaker than that of S. meleagris medusa of which the variation in toxin expressions is feasibly an important reason.
AB - Background: The incidents of Aurelia sp. stinging have recently increased because of a bloom in offshore area. However, their symptoms are much milder than those from another scyphozoan jellyfish, Stomolophus meleagris. Methods: The molecular composition of the medusa and polyp of Aurelia coerulea was analyzed by sequencing the transcriptome and proteome. The toxicity of tentacle extract from A. coerulea medusa (A-TE) and S. meleagris medusa (S-TE) was measured by the survival rates of mice, their blood indexes, and integrity of red blood cells. Results: The medusa and polyp of A. coerulea are similar in molecular composition, while their gene expressions are significantly different at both transcriptome and proteome levels. A-TE displayed no in vitro hemolysis and caused mild damage to the liver, heart and kidney instead of lethality. In contrast, S-TE showed strong hemolytic toxicity, and lethal effect with serious damage to the liver, heart and kidney. The toxin screening in the medusae showed that there were similar toxin categories though the number of toxin species in A. coerulea was larger than that in S. meleagris. Among them, lactotransferrin and venom prothrombin activator were the two predominant protein toxins in the medusae of A. coerulea and S. meleagris, respectively. Conclusions: A. coerulea medusa and polyp have similar molecular compositions, though there are observable morphological differences. The toxicity of A. coerulea medusa is significantly weaker than that of S. meleagris medusa of which the variation in toxin expressions is feasibly an important reason.
KW - Aurelia coerulea
KW - Proteome
KW - Stomolophus meleagris
KW - Toxicity
KW - Toxin
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85125126306&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2022.02.006
DO - 10.1016/j.toxicon.2022.02.006
M3 - Article
C2 - 35183571
AN - SCOPUS:85125126306
VL - 210
SP - 89
EP - 99
JO - Toxicon
JF - Toxicon
SN - 0041-0101
ER -