TY - JOUR
T1 - The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration
AU - Uarrota, Virgílio Gavicho
AU - Moresco, Rodolfo
AU - Schmidt, Eder Carlos
AU - Bouzon, Zenilda Laurita
AU - Da Costa Nunes, Eduardo
AU - De Oliveira Neubert, Enilto
AU - Peruch, Luiz Augusto Martins
AU - Rocha, Miguel
AU - Maraschin, Marcelo
N1 - Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2016/4/15
Y1 - 2016/4/15
N2 - This study aimed to investigate the role of ascorbate peroxidase (APX), guaiacol peroxidase (GPX), polysaccharides, and protein contents associated with the early events of postharvest physiological deterioration (PPD) in cassava roots. Increases in APX and GPX activity, as well as total protein contents occurred from 3 to 5 days of storage and were correlated with the delay of PPD. Cassava samples stained with Periodic Acid-Schiff (PAS) highlighted the presence of starch and cellulose. Degradation of starch granules during PPD was also detected. Slight metachromatic reaction with toluidine blue is indicative of increasing of acidic polysaccharides and may play an important role in PPD delay. Principal component analysis (PCA) classified samples according to their levels of enzymatic activity based on the decision tree model which showed GPX and total protein amounts to be correlated with PPD. The Oriental (ORI) cultivar was more susceptible to PPD.
AB - This study aimed to investigate the role of ascorbate peroxidase (APX), guaiacol peroxidase (GPX), polysaccharides, and protein contents associated with the early events of postharvest physiological deterioration (PPD) in cassava roots. Increases in APX and GPX activity, as well as total protein contents occurred from 3 to 5 days of storage and were correlated with the delay of PPD. Cassava samples stained with Periodic Acid-Schiff (PAS) highlighted the presence of starch and cellulose. Degradation of starch granules during PPD was also detected. Slight metachromatic reaction with toluidine blue is indicative of increasing of acidic polysaccharides and may play an important role in PPD delay. Principal component analysis (PCA) classified samples according to their levels of enzymatic activity based on the decision tree model which showed GPX and total protein amounts to be correlated with PPD. The Oriental (ORI) cultivar was more susceptible to PPD.
KW - Antioxidant enzymes
KW - Cassava roots
KW - L-ascorbic acid peroxidase
KW - Polysaccharides
KW - Postharvest
KW - Postharvest
UR - http://www.scopus.com/inward/record.url?scp=84946781015&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2015.11.025
DO - 10.1016/j.foodchem.2015.11.025
M3 - Article
C2 - 26617011
AN - SCOPUS:84946781015
SN - 0308-8146
VL - 197
SP - 737
EP - 746
JO - Food Chemistry
JF - Food Chemistry
ER -