TY - JOUR
T1 - Volatile profiles of human skin cell cultures in different degrees of senescence
AU - Acevedo, Cristian A.
AU - Sanchez, Elizabeth Y.
AU - Reyes, Juan G.
AU - Young, Manuel E.
PY - 2010/2/1
Y1 - 2010/2/1
N2 - It is known that skin releases volatile organic compounds to the environment, and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, a simple and non-destructive method consisting of SPME sampling and GC/MS analysis was developed to identify volatile organic emanations from cell cultures. This technique, applied to skin cells culture, indicates that the cells or cell metabolism produce several skin emissions. Chemometric analysis was performed in order to explore the relationship between a volatile profile and the senescence of cell cultures. Volatile profiles were different for cell cultures in different degrees of senescence, indicating that volatile compound patterns could be used to provide information about the age of skin cells.
AB - It is known that skin releases volatile organic compounds to the environment, and also that its emission pattern changes with aging of the skin. It could be considered, that these compounds are intermediaries in cell metabolism, since many intermediaries of metabolic pathways have a volatile potential. In this work, a simple and non-destructive method consisting of SPME sampling and GC/MS analysis was developed to identify volatile organic emanations from cell cultures. This technique, applied to skin cells culture, indicates that the cells or cell metabolism produce several skin emissions. Chemometric analysis was performed in order to explore the relationship between a volatile profile and the senescence of cell cultures. Volatile profiles were different for cell cultures in different degrees of senescence, indicating that volatile compound patterns could be used to provide information about the age of skin cells.
KW - SPME
KW - Senescence
KW - Skin cell
KW - VOC
UR - http://www.scopus.com/inward/record.url?scp=74049093656&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2009.12.033
DO - 10.1016/j.jchromb.2009.12.033
M3 - Article
C2 - 20061192
AN - SCOPUS:74049093656
VL - 878
SP - 449
EP - 455
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 3-4
ER -