TY - JOUR
T1 - Water as Cosolvent
T2 - Nonviscous Deep Eutectic Solvents for Efficient Lipase-Catalyzed Esterifications
AU - Guajardo, Nadia
AU - Domínguez de María, Habil Pablo
AU - Ahumada, Katherine
AU - Schrebler, Rodrigo A.
AU - Ramírez-Tagle, Rodrigo
AU - Crespo, Fernando A.
AU - Carlesi, Carlos
N1 - Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/4/21
Y1 - 2017/4/21
N2 - The use of deep eutectic solvent (DES)/water mixtures were explored for the selective enzymatic synthesis of α-monobenzoate glycerol (α-MBG) from glycerol and benzoic acid as substrates. Experiments were performed with four DES, three of them containing choline chloride (ChCl), combined with urea (URA), glycerol (GLY), and ethylene glycol (ETA) (in all cases ChCl/HBD 1:2 mol ratio), and another one formed with methylammonium chloride and glycerol (MA/GLY 1:3 mol ratio). The best conversions (99 %) were achieved with immobilized lipase B from Candida antarctica (CAL-B) when ChCl/GLY was used as the solvent and the substrate at the same time. The use of water as a cosolvent (8 % v/v) led to a significant decrease in the viscosity of the DES, and full conversions were then reached. Reusability studies of the biocatalyst revealed a 37 % decrease in activity after the first batch, but the activity remained mostly constant for the rest of the cycles.
AB - The use of deep eutectic solvent (DES)/water mixtures were explored for the selective enzymatic synthesis of α-monobenzoate glycerol (α-MBG) from glycerol and benzoic acid as substrates. Experiments were performed with four DES, three of them containing choline chloride (ChCl), combined with urea (URA), glycerol (GLY), and ethylene glycol (ETA) (in all cases ChCl/HBD 1:2 mol ratio), and another one formed with methylammonium chloride and glycerol (MA/GLY 1:3 mol ratio). The best conversions (99 %) were achieved with immobilized lipase B from Candida antarctica (CAL-B) when ChCl/GLY was used as the solvent and the substrate at the same time. The use of water as a cosolvent (8 % v/v) led to a significant decrease in the viscosity of the DES, and full conversions were then reached. Reusability studies of the biocatalyst revealed a 37 % decrease in activity after the first batch, but the activity remained mostly constant for the rest of the cycles.
KW - biocatalysis
KW - deep eutectic solvents
KW - enzymes
KW - esterification
KW - solvent effects
UR - http://www.scopus.com/inward/record.url?scp=85017141225&partnerID=8YFLogxK
U2 - 10.1002/cctc.201601575
DO - 10.1002/cctc.201601575
M3 - Article
AN - SCOPUS:85017141225
SN - 1867-3880
VL - 9
SP - 1393
EP - 1396
JO - ChemCatChem
JF - ChemCatChem
IS - 8
ER -