Water as Cosolvent: Nonviscous Deep Eutectic Solvents for Efficient Lipase-Catalyzed Esterifications

Nadia Guajardo, Habil Pablo Domínguez de María, Katherine Ahumada, Rodrigo A. Schrebler, Rodrigo Ramírez-Tagle, Fernando A. Crespo, Carlos Carlesi

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The use of deep eutectic solvent (DES)/water mixtures were explored for the selective enzymatic synthesis of α-monobenzoate glycerol (α-MBG) from glycerol and benzoic acid as substrates. Experiments were performed with four DES, three of them containing choline chloride (ChCl), combined with urea (URA), glycerol (GLY), and ethylene glycol (ETA) (in all cases ChCl/HBD 1:2 mol ratio), and another one formed with methylammonium chloride and glycerol (MA/GLY 1:3 mol ratio). The best conversions (99 %) were achieved with immobilized lipase B from Candida antarctica (CAL-B) when ChCl/GLY was used as the solvent and the substrate at the same time. The use of water as a cosolvent (8 % v/v) led to a significant decrease in the viscosity of the DES, and full conversions were then reached. Reusability studies of the biocatalyst revealed a 37 % decrease in activity after the first batch, but the activity remained mostly constant for the rest of the cycles.

Original languageEnglish
Pages (from-to)1393-1396
Number of pages4
JournalChemCatChem
Volume9
Issue number8
DOIs
StatePublished - 21 Apr 2017
Externally publishedYes

Keywords

  • biocatalysis
  • deep eutectic solvents
  • enzymes
  • esterification
  • solvent effects

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