Ácido p-cumárico reduce el deterioro mediado por alta glucosa de la relajación dependiente de endotelio en aorta de rata

Raúl Vinet, Patricio Araos, Juan Carlos Gentina, Marcela Knox, Leda Guzmán

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

7 Citas (Scopus)

Resumen

p-Coumaric acid (p-CA) is an ubiquitous plant metabolite with antioxidant, anti-inflammatory, and anticancer properties. The present study was designed to evaluate the preventive effects of p-CA on endothelium-dependent impairment produced by high glucose (HG) (D-Glucose 25 mM) in isolated rat thoracic aorta. Aortic rings obtained from male Sprague-Dawley rats were mounted in an organ bath and pretreated for 3 h with D-Glucose 5 mM, HG and HG plus p-CA (1, 10 and 100 μM). After this period of time endotheliumdependent relaxation was tested by cumulative addition of acetylcholine (ACh) in pre-contracted rings with phenylephrine (PE) (0.1 μM). P-CA elicited a moderate endothelium-dependent vasodilatory effect (Emax= 29.28 ± 1.89%, N=6; pD2= 6.075 ± 0.184, N=6). When aortic rings were pre-incubated for 3 h with HG, Emax for ACh decreased dramatically from 87.69 ± 2.59% (N=6) to 40.54 ± 1.78% (N=6). The negative effect of HG was partially reverted in rings co-incubated with p-CA in a concentration-dependent manner as shown for Emax values to each p-CA concentration: 1 μM (48.57 ± 1.82%), 10 μM (60.81 ± 1.80%) and 100 μM (64.51 ± 1.80%). The action of p-CA was associated with a significant change in Emax. No statistical difference in pD2 was observed. Our results clearly show that p-CA protect ACh-induced endothelial-dependent relaxation affected by HG in isolated rat aortic rings.

Título traducido de la contribuciónp-Coumaric acid reduces high glucose-mediated impairment of endothelium-dependent relaxation in rat aorta
Idioma originalEspañol
Páginas (desde-hasta)232-237
Número de páginas6
PublicaciónBoletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas
Volumen13
N.º3
EstadoPublicada - may. 2014
Publicado de forma externa

Palabras clave

  • Acetylcholine
  • Endothelium
  • High glucose
  • Rat aorta
  • p-coumaric acid

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